Battistel Dario, Baldi Franco, Gallo Michele, Faleri Claudia, Daniele Salvatore
Dipartimento di Scienze Ambientali, Infomatica e Statistica, University Cà Foscari Venice. Calle Larga Santa Marta 2137. 30127 Venice, (I).
Dipartimento di Scienze Molecolari e Nanosistemi, University Cà Foscari Venice. Calle Larga Santa Marta 2137. 30127 Venice, (I).
Talanta. 2015 Jan;132:294-300. doi: 10.1016/j.talanta.2014.09.023. Epub 2014 Sep 19.
Silver nanoparticles (AgNPs) were biosynthesised by a Klebsiella oxytoca strain BAS-10, which, during its growth, is known to produce a branched exopolysaccharide (EPS). Klebsiella oxytoca cultures, treated with AgNO3 and grown under either aerobic or anaerobic conditions, produced silver nanoparticles embedded in EPS (AgNPs-EPS) containing different amounts of Ag(0) and Ag(I) forms. The average size of the AgNPs-EPS was determined by transmission electron microscopy, while the relative abundance of Ag(0)- or Ag(I)-containing AgNPs-EPS was established by scanning electrochemical microscopy (SECM). Moreover, the release of silver(I) species from the various types of AgNPs-EPS was investigated by combining SECM with anodic stripping voltammetry. These measurements allowed obtaining information on the kinetic of silver ions release from AgNPs-EPS and their concentration profiles at the substrate/water interface.
银纳米颗粒（AgNPs）由产酸克雷伯菌BAS-10菌株生物合成，该菌株在生长过程中已知会产生一种分支胞外多糖（EPS）。用硝酸银处理并在有氧或厌氧条件下培养的产酸克雷伯菌培养物产生了包埋在EPS中的银纳米颗粒（AgNPs-EPS），其中含有不同量的Ag(0)和Ag(I)形式。通过透射电子显微镜测定AgNPs-EPS的平均尺寸，而通过扫描电化学显微镜（SECM）确定含Ag(0)或Ag(I)的AgNPs-EPS的相对丰度。此外，通过将SECM与阳极溶出伏安法相结合，研究了各种类型的AgNPs-EPS中银(I)物种的释放。这些测量使得能够获得关于银离子从AgNPs-EPS释放的动力学及其在底物/水界面处的浓度分布的信息。
