Kejriwal Swati, Bhandary Rahul, Thomas Biju, Kumari Suchetha
Postgraduate Student, Department of Periodontics, A.B.Shetty Memorial Institute of Dental Sciences , Mangalore, India .
Professor, Department of Periodontics, A. B. Shetty Memorial Institute of Dental Sciences , Mangalore, India .
J Clin Diagn Res. 2014 Oct;8(10):ZC56-60. doi: 10.7860/JCDR/2014/8239.5042. Epub 2014 Oct 20.
Periodontal diseases are a group of inflammatory conditions resulting from interaction between a pathogenic bacterial biofilm and susceptible host's inflammatory response eventually leading to the destruction of periodontal structures and subsequent tooth loss. Hence, investigation of salivary proteins in individuals with periodontal diseases may be useful to enhance the knowledge of their roles in these diseases.
This case-control study was conducted at A.B. Shetty Memorial Institute of Dental Sciences, Mangalore. The study comprised of 90 patients of age between 25-60 years who were clinically examined and divided into three groups of 30 each: namely clinically healthy, gingivitis and chronic periodontitis. These were classified according to the values of gingival index score, clinical attachment loss and probing pocket depth. Unstimulated saliva was collected and salivary mucin, amylase and total protein levels were determined.
RESULTS obtained were tabulated and statistically analyzed using ANOVA test and Karl pearson's correlation test.
The results of the study showed an increased concentration of salivary mucin, amylase and total protein in gingivitis patients and increased levels of amylase and total protein in saliva of chronic periodontitis patients compared to healthy individuals which were statistically significant. A decrease in mucin concentration was observed in the periodontitis group compared to gingivitis group. A positive correlation was present between salivary mucin, amylase and total protein levels in the three groups.
Salivary mucin, amylase and total protein may serve as an important biochemical parameter of inflammation of the periodontium. Also, it can be hypothesized that various enzyme inhibitors might be useful as a part of host modulation therapy in the treatment of periodontal diseases.
牙周疾病是一组炎症性病症，由致病性细菌生物膜与易感宿主的炎症反应相互作用引起，最终导致牙周结构破坏及随后的牙齿脱落。因此，研究牙周疾病患者的唾液蛋白可能有助于增进对其在这些疾病中作用的了解。
本病例对照研究在芒格洛尔的A.B.谢蒂纪念牙科学院进行。该研究包括90名年龄在25至60岁之间的患者，这些患者经过临床检查并分为三组，每组30人：即临床健康组、牙龈炎组和慢性牙周炎组。这些组是根据牙龈指数评分、临床附着丧失和探诊袋深度的值进行分类的。收集未刺激唾液并测定唾液粘蛋白、淀粉酶和总蛋白水平。
将获得的结果制成表格，并使用方差分析测试和卡尔·皮尔逊相关性测试进行统计分析。
研究结果显示，与健康个体相比，牙龈炎患者唾液粘蛋白、淀粉酶和总蛋白浓度升高，慢性牙周炎患者唾液中淀粉酶和总蛋白水平升高，且具有统计学意义。与牙龈炎组相比，牙周炎组粘蛋白浓度降低。三组唾液粘蛋白、淀粉酶和总蛋白水平之间存在正相关。
唾液粘蛋白、淀粉酶和总蛋白可能是牙周组织炎症的重要生化参数。此外，可以推测各种酶抑制剂可能作为宿主调节疗法的一部分用于治疗牙周疾病。
