Bhavana Sujanamulk, Nallamilli Sai Madhavi, Thippani Maneshwar, Gundapaneni Namratha, Sahitya Yamini, Keerthi Reddy Vummadi, Dey Subhra, Takkella Bharani Krishna, Prabhat Mp Venkata, Venkata Anusha Nayanala
Oral Medicine and Radiology, Drs Sudha and Nageswara Rao Siddhartha Institute of Dental Sciences, Vijayawada, IND.
Oral Medicine and Radiology, Mahaveer Institute Of Medical Sciences, Vikarabad, IND.
Cureus. 2023 Dec 17;15(12):e50654. doi: 10.7759/cureus.50654. eCollection 2023 Dec.
Long-term use of chemical plaque control methods has led to adverse effects. In the current scenario, herbal mouthwashes have adequately remediated periodontal disease. Moreover, in the salivary interactome, biomarkers such as salivary amylase, a key protein secreted in the saliva, have been immensely useful in detecting the progression of periodontal disease. Therefore, we aimed to determine the anti-inflammatory effect of ethanolic crude extracts of leaf and peel and to estimate salivary amylase levels in subjects diagnosed with chronic periodontitis.
Ethanolic extracts of both plants were prepared using the soxhlet extraction method, and the obtained metabolites were confirmed by thin-layer chromatography. After a mouthwash was prepared, 100 subjects were randomly divided into two groups. Group I received peel (Pgp) mouthwash, and Group II received leaf (Wfl). Clinical parameters such as probing depth and clinical attachment loss were recorded to determine the grades of periodontitis. Unstimulated whole saliva was collected, and amylase levels were analyzed at three-time intervals using spectrophotometric assay.
For both groups, the mean reduction in salivary amylase levels was calculated at baseline after 1 hour and 1 week of using the mouthwash. A statistically highly significant reduction was seen with (p=0.000*) at the 1-hr interval in Group I.
The anti-inflammatory effect was more effective with Pgp mouthwash when compared to Wfl mouthwash.
长期使用化学菌斑控制方法已导致不良反应。在当前情况下，草药漱口水已充分改善了牙周疾病。此外，在唾液相互作用组中，诸如唾液淀粉酶（唾液中分泌的一种关键蛋白质）等生物标志物在检测牙周疾病的进展方面非常有用。因此，我们旨在确定叶和果皮乙醇粗提物的抗炎作用，并估计被诊断为慢性牙周炎的受试者的唾液淀粉酶水平。
使用索氏提取法制备两种植物的乙醇提取物，并通过薄层色谱法确认所得代谢物。制备漱口水后，将100名受试者随机分为两组。第一组接受果皮漱口水（Pgp），第二组接受叶漱口水（Wfl）。记录诸如探诊深度和临床附着丧失等临床参数以确定牙周炎的等级。收集未刺激的全唾液，并使用分光光度法在三个时间间隔分析淀粉酶水平。
对于两组，在使用漱口水1小时和1周后的基线时计算唾液淀粉酶水平的平均降低。第一组在1小时间隔时观察到统计学上高度显著的降低（p = 0.000*）。
与Wfl漱口水相比，Pgp漱口水的抗炎效果更有效。
