Sundar N Mani, Krishnan V, Krishnaraj S, Hemalatha V T, Alam Md Nazish
Assistant Professor,Department of Periodontics, Sree Balaji Dental College , Chennai, India .
J Clin Diagn Res. 2013 Jun;7(6):1223-7. doi: 10.7860/JCDR/2013/5386.3068. Epub 2013 Jun 1.
Nitric oxide (NO) is a ubiquitous intercellular messenger molecule with important cardiovascular, neurological, and immune functions. In addition, it has been postulated that the pharmacological inhibition of NO or its actions may be therapeutically valuable in the disease management. The levels of nitric oxide may provide clues about the severity and the state of the underlying disease process. It could be an inflammatory biomarker that may enable clinicians to direct the environmentally based prevention or treatment programmes and to establish whether NO plays a role in the pathogenesis of periodontitis or not. Hence, the aim of the present study was to evaluate the salivary and the serum levels of NO in generalized chronic and aggressive periodontitis. The Study Design: Unstimulated whole saliva and serum samples were collected from a total of 60 subjects who were in the age group of 18-45 years, who participated in this study. They were divided into three equal groups with 20 subjects in each group; group A (healthy controls), group B (chronic periodontitis) and group C (aggressive periodontitis). The clinical parameters were assessed, based on the oral hygiene index simplified (OHI-S), the gingival index (GI), the probing pocket depth and the clinical attachment loss (CAL). A biochemical analysis was performed to evaluate and compare the salivary and the serum nitric oxide levels of the above groups. Statistical Analysis and Results: The statistical comparisons were done under the Griess Reaction. There were statistically significant salivary and serum levels of NO in the groups of periodontitis (group B and C) as compared to those in the healthy controls (group A). A significant positive correlation was found between the values of the salivary and the serum NO levels in chronic and aggressive periodontitis.
Nitric oxide is a potent modulator of the inflammatory disease processes and under pathological conditions, NO has damaging effects. As there is a paucity in the studies which have compared chronic and aggressive periodontitis, this study paved an interest for combining the serum and the salivary analysis in comparing the levels of nitric oxide in chronic and aggressive periodontitis.
一氧化氮（NO）是一种普遍存在的细胞间信使分子，具有重要的心血管、神经和免疫功能。此外，据推测，对NO或其作用进行药理抑制在疾病管理中可能具有治疗价值。一氧化氮水平可能为潜在疾病进程的严重程度和状态提供线索。它可能是一种炎症生物标志物，使临床医生能够指导基于环境的预防或治疗方案，并确定NO是否在牙周炎的发病机制中起作用。因此，本研究的目的是评估广泛性慢性和侵袭性牙周炎患者唾液和血清中的NO水平。
从总共60名年龄在18 - 45岁之间参与本研究的受试者中收集未刺激的全唾液和血清样本。他们被分为三组，每组20名受试者；A组（健康对照组）、B组（慢性牙周炎组）和C组（侵袭性牙周炎组）。根据简化口腔卫生指数（OHI-S）、牙龈指数（GI）、探诊袋深度和临床附着丧失（CAL）评估临床参数。进行生化分析以评估和比较上述各组的唾液和血清一氧化氮水平。
在格里斯反应（Griess Reaction）下进行统计比较。与健康对照组（A组）相比，牙周炎组（B组和C组）的唾液和血清NO水平具有统计学意义。在慢性和侵袭性牙周炎中，唾液和血清NO水平值之间发现显著正相关。
一氧化氮是炎症疾病进程的有效调节剂，在病理条件下，NO具有破坏作用。由于比较慢性和侵袭性牙周炎的研究较少，本研究为结合血清和唾液分析来比较慢性和侵袭性牙周炎中一氧化氮水平引发了兴趣。
