Koshte V L, Kagen S L, Aalberse R C
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
J Allergy Clin Immunol. 1989 Aug;84(2):174-83. doi: 10.1016/0091-6749(89)90322-9.
We investigated the possibility that subjects with IgE antibodies to an inhalant insect allergen, such as caddis fly, might also have antibodies to cross-reacting carbohydrate determinants (CCDs). IgE antibodies to cross-reacting allergens in caddis flies, mussels, oysters, shrimps, crabs, honeybee, and yellow jacket venoms were determined by RAST, RAST inhibition, and immunoblot studies with sera from three different sources: (1) sera of patients with well-defined inhalant atopy to caddis fly, (2) sera with IgE anti-CCD antibodies from subjects without known exposure to caddis fly, and (3) hyperimmune antisera with IgG anti-CCD antibodies raised as a result of immunization of rabbits with grass-pollen extract, buckwheat glycoprotein, or with honeybee venom. Sera from groups 2 and 3 reacted with Sepharose-coupled caddis fly extract in a RAST-type assay and elicited virtually identical patterns on immunoblots of caddis fly extract separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, whereas the sera from group 1 atopic patients did not react with CCD-rich material. However, indications for other types of cross-reacting antibodies were detected. The IgE antibodies of one of the patients studied (who was allergic not only to caddis fly but also to shellfish) were found to detect a cross-reacting homologous protein in extracts of mussel, oyster, shrimp, crab, honeybee, and yellow jacket venom. Preliminary results suggest that this cross-reacting 13 kd protein, the most prominent caddis fly allergen, is an invertebrate hemoglobin (erythrocruorin)-like molecule. These studies suggest the possibility that patients sensitized by exposure to caddis fly antigens could develop allergic reactions during their first exposure to shellfish or to their first bee sting.
我们研究了这样一种可能性，即对吸入性昆虫过敏原（如毛翅目昆虫）具有IgE抗体的受试者，可能也具有针对交叉反应性碳水化合物决定簇（CCDs）的抗体。通过放射变应原吸附试验（RAST）、RAST抑制试验以及免疫印迹研究，测定了来自三种不同来源血清中针对毛翅目昆虫、贻贝、牡蛎、虾、蟹、蜜蜂和黄蜂毒液中交叉反应性过敏原的IgE抗体：（1）对毛翅目昆虫有明确吸入性特应性的患者血清；（2）来自未知接触毛翅目昆虫的受试者的具有IgE抗CCDs抗体的血清；（3）由于用草花粉提取物、荞麦糖蛋白或蜜蜂毒液免疫兔子而产生的具有IgG抗CCDs抗体的超免疫抗血清。在RAST类型的试验中，第2组和第3组的血清与琼脂糖偶联的毛翅目昆虫提取物发生反应，并且在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的毛翅目昆虫提取物的免疫印迹上产生几乎相同的条带模式，而第1组特应性患者的血清不与富含CCDs的物质发生反应。然而，检测到了其他类型交叉反应抗体的迹象。在研究的一名患者（不仅对毛翅目昆虫过敏，而且对贝类过敏）的IgE抗体中，发现其能检测到贻贝、牡蛎、虾、蟹、蜜蜂和黄蜂毒液提取物中的一种交叉反应同源蛋白。初步结果表明，这种交叉反应的13 kd蛋白，即最主要的毛翅目昆虫过敏原，是一种类似无脊椎动物血红蛋白（红血球素）的分子。这些研究提示，因接触毛翅目昆虫抗原而致敏的患者在首次接触贝类或首次被蜜蜂蜇刺时可能发生过敏反应。
