Li Zhanfeng, Zhang Cong, Wang Bingnan, Wang Hongyan, Chen Xuesi, Möhwald Helmoth, Cui Xuejun
College of Chemistry, Jilin University , Changchun, 130012, P. R. China.
ACS Appl Mater Interfaces. 2014 Dec 24;6(24):22166-73. doi: 10.1021/am5057097. Epub 2014 Dec 5.
In the present study, the molecular and magnetic dual-targeted redox-responsive folic acid-cysteine-Fe3O4 microcapsules (FA-Cys-Fe3O4 MCs) have been synthesized via the sonochemical technique, and targeting molecule (folic acid) and Fe3O4 magnetic nanoparticles are introduced into the microcapsule shells successfully. The obtained FA-Cys-Fe3O4 MCs show excellent magnetic responsive ability by the oriented motion under an external magnetic field. The hydrophobic fluorescent dye (Coumarin 6) is successfully loaded into the FA-Cys-Fe3O4 MCs, demonstrating that it could be also easily realized to encapsulate hydrophobic drugs into the FA-Cys-Fe3O4 MCs when the drugs are dispersed into the oil phase before sonication. Cellular uptake demonstrates that FA-Cys-Fe3O4 MCs could target selectively the cells via folate-receptor-mediated endocytosis. Moreover, the FA-Cys-Fe3O4 MCs show their potential ability to be an attractive carrier for drug controlled release owing to the redox responsiveness of the disulfide in the microcapsule shells.
在本研究中，通过声化学技术合成了分子与磁性双靶向的氧化还原响应型叶酸 - 半胱氨酸 - Fe3O4微胶囊（FA - Cys - Fe3O4 MCs），并将靶向分子（叶酸）和Fe3O4磁性纳米粒子成功引入微胶囊壳层。所制备的FA - Cys - Fe3O4 MCs在外部磁场作用下通过定向运动表现出优异的磁响应能力。疏水性荧光染料（香豆素6）成功负载到FA - Cys - Fe3O4 MCs中，这表明当药物在超声处理前分散到油相中时，也能够轻松地将疏水性药物封装到FA - Cys - Fe3O4 MCs中。细胞摄取实验表明，FA - Cys - Fe3O4 MCs能够通过叶酸受体介导的内吞作用选择性地靶向细胞。此外，由于微胶囊壳层中二硫键的氧化还原响应性，FA - Cys - Fe3O4 MCs显示出作为药物控释有吸引力载体的潜在能力。
