Kämpfer Peter, Jerzak Leszek, Wilharm Gottfried, Golke Jan, Busse Hans-Jürgen, Glaeser Stefanie P
Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Faculty of Biological Sciences, University of Zielona Góra, Poland.
Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):784-788. doi: 10.1099/ijs.0.000014. Epub 2014 Dec 5.
A Gram-stain-positive bacterial isolate, strain 280/10(T) was isolated from the trachea of a white stork. The isolate was morphologically rod- to coccoid-shaped, non-motile and showed no oxidase activity. The highest 16S rRNA gene sequence similarity was found to the type strain of Corynebacterium ciconiae (97.3 % similarity) as the nearest relative of strain 280/10(T), all other 16S rRNA gene sequence similarities to type strains of species of the genus Corynebacterium were below 94.2 %. Strain 280/10(T) had a quinone system consisting predominantly of menaquinone MK-8(H2), minor quantities of MK-9(H2) and small amounts of MK-8. The diamino acid of the peptidoglycan was meso-diaminopimelic acid. The major compounds in the polar lipid profiles were diphosphatidylglycerol, phoshatidylglycerol, phosphatidylinositol and an acidic glycolipid. Two phosphatidylinositol-mannosides and several unidentified lipids were also present. The strain contained corynemycolic acids, while only small amounts of cellular polyamines were detected. Spermidine and spermine were predominant in the polyamine pattern of 280/10(T) and putrescine was present in moderate amounts. In the fatty acid profile C18 : 1ω9c, and C16 : 0 were predominant. The strain was distinguishable from C. ciconiae, which is the most closely related species. In conclusion, strain 280/10(T) is proposed to represent a novel species of the genus Corynebacterium with the name Corynebacterium trachiae sp. nov. and the type strain 280/10(T) ( = CIP 110796(T) = LMG 28214(T)).
从一只白鹳的气管中分离出一株革兰氏阳性细菌,菌株280/10(T)。该分离株形态上呈杆状至球状,无运动性,且无氧化酶活性。16S rRNA基因序列与鹳棒杆菌模式菌株的相似度最高(相似性为97.3%),是菌株280/10(T)最近的亲缘关系,与棒杆菌属其他物种模式菌株的所有其他16S rRNA基因序列相似性均低于94.2%。菌株280/10(T)的醌系统主要由甲基萘醌MK-8(H2)组成,少量的MK-9(H2)和微量的MK-8。肽聚糖的二氨基酸是内消旋二氨基庚二酸。极性脂质谱中的主要化合物是双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和一种酸性糖脂。还存在两种磷脂酰肌醇甘露糖苷和几种未鉴定的脂质。该菌株含有棒杆菌酸,而仅检测到少量细胞多胺。亚精胺和精胺在280/10(T)的多胺模式中占主导地位,腐胺含量适中。在脂肪酸谱中,C18:1ω9c和C16:0占主导地位。该菌株与最密切相关的物种鹳棒杆菌不同。总之,提议菌株280/10(T)代表棒杆菌属的一个新物种,命名为气管棒杆菌新种,模式菌株为280/10(T)(=CIP 110796(T)=LMG 28214(T))。