Wei Yuli, Fang Jiasong, Xu Yunping, Zhao Weiqiang, Cao Junwei
Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, PR China.
National Engineering Research Centre for Oceanic Fisheries, Shanghai Ocean University, Shanghai 201306, PR China.
Int J Syst Evol Microbiol. 2018 May;68(5):1474-1478. doi: 10.1099/ijsem.0.002695. Epub 2018 Mar 20.
A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6, was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically. Optimal bacterial growth occurred at 35 °C (range 22-41 °C), at pH 6 (4-8) and with 4 % (w/v) NaCl (0-10 %). The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6 belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains. The major respiratory menaquinones were MK-8(H2) and MK-9(H2). The polar lipids were diphosphatidyglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified aminoglycolipids and four unidentified glycolipids. The major fatty acids detected were C18 : 1ω9c, C16 : 0 and C15 : 0. Strain NBT06-6 contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars. The G+C content of the genomic DNA was 65.1 mol%. The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6 and C. imitans were 24.5±2.4 and 81.9 %, respectively. The combined genotypic and phenotypic data indicated that strain NBT06-6 represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp. nov. is proposed, with the type strain NBT06-6 (=MCCC 1K03347=DSM 105365).
从新不列颠海沟的深层海水中分离出一株新型异养、革兰氏染色阳性、兼性厌氧的杆状细菌，命名为菌株NBT06-6，并对其进行了系统发育和表型特征分析。该菌株在35°C（范围为22-41°C）、pH值为6（4-8）、NaCl含量为4%（w/v）（0-10%）的条件下生长最佳。在35°C时，该菌株能在0.1-100 MPa的静水压力下生长（最适压力为0.1 MPa）。对16S rRNA基因序列的系统发育分析表明，菌株NBT06-6属于棒状杆菌属，与模仿棒状杆菌的序列相似性最高（97.9%），与其他模式菌株的16S rRNA基因序列相似性较低（<97.0%）。主要呼吸醌为MK-8(H2)和MK-9(H2)。极性脂质为双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、三种未鉴定的氨基糖脂和四种未鉴定的糖脂。检测到的主要脂肪酸为C18:1ω9c、C16:0和C15:0。菌株NBT06-6的细胞壁含有内消旋二氨基庚二酸和分枝菌酸，主要全细胞糖为甘露糖、半乳糖、葡萄糖、阿拉伯糖和核糖。基因组DNA的G+C含量为65.1 mol%。菌株NBT06-6与模仿棒状杆菌之间的数字DNA-DNA杂交值和平均核苷酸同一性分别为24.5±2.4和81.9%。综合基因型和表型数据表明，菌株NBT06-6代表棒状杆菌属的一个新物种，为此提出新名称哈氏棒状杆菌（Corynebacterium hadale sp. nov.），模式菌株为NBT06-6（=MCCC 1K03347=DSM 105365）。
