Li Yan-Xuan, Yang Song-Zhen, Feng Guang-Da, Wang Yong-Hong, Zhu Hong-Hui
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2016 Aug;66(8):3201-3206. doi: 10.1099/ijsem.0.001177. Epub 2016 May 23.
A novel Gram-reaction-positive, non-motile and facultatively anaerobic bacterium, designated strain S01T, was isolated from a nutrient agar plate kept on a laboratory clean bench at Guangdong Institute of Microbiology, PR China, which was contaminated from an unknown source. Strain S01T was found to be catalase-positive and oxidase-negative. Similarity searches revealed that the strain shared the highest 16S rRNA gene similarity with Corynebacterium humireducens MFC-5T (95.9 %). However, phylogenetic analysis based on the 16S rRNA gene sequences showed that strain S01T was closely related to Corynebacteriumdoosanense JCM 17317T (94.8 %) and Corynebacterium maris JCM 17018T (94.8 %). The major fatty acids were C18:1ω9c, C16:0, 10-methyl C18:0 and C18:0. The respiratory quinones predominantly consisted of MK-8(H2), with small amounts of MK-8 and MK-9(H2). Polar lipids contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, an unidentified aminolipid, two unidentified glycolipids and two unidentified lipids. Mycolic acids were present. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major cell-wall sugars were galactose, arabinose and glucose. The genomic DNA G+C content of strain S01T was 70.7±0.1 mol%. The results of phenotypic, phylogenetic and chemotaxonomic analyses indicated that strain S01T represents a novel species of the genus Corynebacterium, for which the name Corynebacterium guangdongense sp. nov. is proposed. The type strain is S01T (=GDMCC 1.1022T=CCTCC AB 2015423T=KCTC 39608T).
从中国广东省微生物研究所实验室超净工作台上一块来源不明的污染营养琼脂平板中，分离出一株新型革兰氏阳性、无运动性兼性厌氧细菌，命名为菌株S01T。发现菌株S01T过氧化氢酶阳性、氧化酶阴性。相似性搜索显示，该菌株与还原土棒杆菌MFC-5T的16S rRNA基因相似度最高（95.9%）。然而，基于16S rRNA基因序列的系统发育分析表明，菌株S01T与斗山棒杆菌JCM 17317T（94.8%）和海棒杆菌JCM 17018T（94.8%）密切相关。主要脂肪酸为C18:1ω9c、C16:0、10-甲基C18:0和C18:0。呼吸醌主要由MK-8(H2)组成，少量为MK-8和MK-9(H2)。极性脂类包含磷脂酰甘油、双磷脂酰甘油、磷脂酰肌醇、一种未鉴定的氨基脂类、两种未鉴定的糖脂类和两种未鉴定的脂类。存在分枝菌酸。细胞壁肽聚糖含有内消旋二氨基庚二酸，主要细胞壁糖类为半乳糖、阿拉伯糖和葡萄糖。菌株S01T的基因组DNA G+C含量为70.7±0.1 mol%。表型、系统发育和化学分类分析结果表明，菌株S01T代表棒杆菌属的一个新物种，为此提出新名称广东棒杆菌。模式菌株为S01T（=GDMCC 1.1022T=CCTCC AB 2015423T=KCTC 39608T）。
