State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Jiangxi Province Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, Nanchang 330006, PR China.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004637.
Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10 and 4HC-13, were isolated from the faeces of greater white-fronted geese () at Poyang Lake, Jiangxi Province, PR China. Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl. The 16S rRNA gene sequences of strains 23H37-10 and 4HC-13 were identical. Phylogenetic and phylogenomic analyses indicated that strains 23H37-10 and 4HC-13 formed an independent cluster within the genus and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to LMM 1652, DSM 7109, DSM 44353 and NCTC 11913, respectively. Cells contained C 9, C and C as the major cellular fatty acids and MK-9 (H) as the predominant respiratory quinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid. Strain 23H37-10 contained mycolic acids, with -diaminopimelic acid and arabinose as the major whole-cell hydrolysates. The genome G+C content of strains 23H37-10 and 4HC-13 was 55.2 mol%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10 and 4HC-13 were 94.4 and 99.6 %, respectively. Strains 23H37-10 and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus , respectively. The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10 and 4HC-13 represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 23H37-10 (=GDMCC 1.1737=KACC 21672).
两株革兰氏阳性、兼性需氧、不运动的杆状到球状的细菌菌株 23H37-10 和 4HC-13 从中国江西省鄱阳湖的大白额雁粪便中分离得到。最佳生长条件为 35-37°C、pH7.0-8.0 和 0.5-1.5%（w/v）NaCl。菌株 23H37-10 和 4HC-13 的 16S rRNA 基因序列完全相同。系统发育和系统基因组分析表明，菌株 23H37-10 和 4HC-13 在 属内形成一个独立的聚类，与 LMM 1652、DSM 7109、DSM 44353 和 NCTC 11913 的 16S rRNA 基因序列相似度分别为 98.8%、97.4%、97.4%和 97.2%。细胞含有 C9、C16:0 和 C18:1ω7c 作为主要的细胞脂肪酸，以及 MK-9 (H) 作为主要的呼吸醌。极性脂由双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、两种未鉴定的磷脂、四种未鉴定的糖脂和一种未鉴定的脂组成。菌株 23H37-10 含有分枝菌酸，以 -二氨基庚二酸和阿拉伯糖作为主要的全细胞水解产物。菌株 23H37-10 和 4HC-13 的基因组 G+C 含量为 55.2mol%。菌株 23H37-10 和 4HC-13 之间的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）值分别为 94.4%和 99.6%。菌株 23H37-10 和 4HC-13 与 属内所有可用基因组的 dDDH 和 ANI 值均小于 70%和 96%。基于表型和生化特征以及差异基因型推断，菌株 23H37-10 和 4HC-13 代表 属内的一个新种，提议命名为 sp. nov.。模式菌株为 23H37-10（=GDMCC 1.1737=KACC 21672）。
