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转录因子Sp1结合位点和八聚体基序在单纯疱疹病毒胸苷激酶基因反式诱导中的作用研究

Examination of the roles of transcription factor Sp1-binding sites and an octamer motif in trans induction of the herpes simplex virus thymidine kinase gene.

作者信息

Böni J, Coen D M

机构信息

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.

出版信息

J Virol. 1989 Sep;63(9):4088-92. doi: 10.1128/JVI.63.9.4088-4092.1989.

DOI:10.1128/JVI.63.9.4088-4092.1989
PMID:2548017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC251013/
Abstract

Herpes simplex virus mutants with both Sp1-binding sites in the thymidine kinase (tk) promoter inactivated or an octamer motif deleted were at most modestly impaired for tk expression. Thus, no cellular transcription factor that binds upstream of the tk TATA box is solely required for trans induction of this gene.

摘要

单纯疱疹病毒突变体中，胸苷激酶（tk）启动子中的两个Sp1结合位点均失活或八聚体基序缺失，其tk表达最多仅受到轻微损害。因此，tk TATA框上游结合的细胞转录因子并非该基因反式诱导所唯一必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deed/251013/2e2b24a43518/jvirol00076-0546-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deed/251013/4a367616dc87/jvirol00076-0546-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deed/251013/2e2b24a43518/jvirol00076-0546-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deed/251013/4a367616dc87/jvirol00076-0546-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deed/251013/2e2b24a43518/jvirol00076-0546-b.jpg

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Examination of the roles of transcription factor Sp1-binding sites and an octamer motif in trans induction of the herpes simplex virus thymidine kinase gene.转录因子Sp1结合位点和八聚体基序在单纯疱疹病毒胸苷激酶基因反式诱导中的作用研究
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本文引用的文献

1
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
2
Generation of genetic diversity in herpes simplex virus: an antimutator phenotype maps to the DNA polymerase locus.单纯疱疹病毒遗传多样性的产生：一种抗突变表型定位于DNA聚合酶基因座。
Virology. 1984 Jan 15;132(1):26-37. doi: 10.1016/0042-6822(84)90088-6.
3
Anti-herpesvirus activity of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine.无环核苷9-(1,3-二羟基-2-丙氧甲基)鸟嘌呤的抗疱疹病毒活性
单纯疱疹病毒胸苷激酶基因中一个不同寻常的内部核糖体进入位点。
Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9667-72. doi: 10.1073/pnas.0504132102. Epub 2005 Jun 22.
4
High-frequency phenotypic reversion and pathogenicity of an acyclovir-resistant herpes simplex virus mutant.一种阿昔洛韦耐药单纯疱疹病毒突变体的高频表型回复和致病性
J Virol. 2003 Feb;77(3):2282-6. doi: 10.1128/jvi.77.3.2282-2286.2003.
5
Identification, localization, and regulation of expression of the UL24 protein of herpes simplex virus type 1.1型单纯疱疹病毒UL24蛋白的鉴定、定位及表达调控
J Virol. 2002 Nov;76(21):10821-8. doi: 10.1128/jvi.76.21.10821-10828.2002.
6
Human thymidine kinase can functionally replace herpes simplex virus type 1 thymidine kinase for viral replication in mouse sensory ganglia and reactivation from latency upon explant.人胸苷激酶可在功能上替代单纯疱疹病毒1型胸苷激酶，用于在小鼠感觉神经节中的病毒复制以及在植入后从潜伏状态重新激活。
J Virol. 1998 Aug;72(8):6710-5. doi: 10.1128/JVI.72.8.6710-6715.1998.
7
Developmental acquisition of enhancer function requires a unique coactivator activity.增强子功能的发育性获得需要独特的共激活因子活性。
EMBO J. 1997 Apr 1;16(7):1721-31. doi: 10.1093/emboj/16.7.1721.
8
Herpes simplex virus infected cell polypeptide 4 preferentially represses Sp1-activated over basal transcription from its own promoter.单纯疱疹病毒感染细胞多肽4优先抑制其自身启动子上Sp1激活的转录，而非基础转录。
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9528-32. doi: 10.1073/pnas.90.20.9528.
9
TATA-dependent enhancer stimulation of promoter activity in mice is developmentally acquired.小鼠中依赖TATA的增强子对启动子活性的刺激是在发育过程中获得的。
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10
Induction of transcription by a viral regulatory protein depends on the relative strengths of functional TATA boxes.病毒调节蛋白对转录的诱导作用取决于功能性TATA框的相对强度。
Mol Cell Biol. 1995 Sep;15(9):4998-5006. doi: 10.1128/MCB.15.9.4998.
Antimicrob Agents Chemother. 1983 May;23(5):676-82. doi: 10.1128/AAC.23.5.676.
4
Unique spectrum of activity of 9-[(1,3-dihydroxy-2-propoxy)methyl]-guanine against herpesviruses in vitro and its mode of action against herpes simplex virus type 1.9-[(1,3-二羟基-2-丙氧基)甲基]鸟嘌呤对疱疹病毒的独特体外活性谱及其对1型单纯疱疹病毒的作用方式
Proc Natl Acad Sci U S A. 1983 May;80(9):2767-70. doi: 10.1073/pnas.80.9.2767.
5
Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus.单纯疱疹病毒胸苷激酶基因转录控制信号之间的功能关系。
Cell. 1982 Dec;31(2 Pt 1):355-65. doi: 10.1016/0092-8674(82)90129-5.
6
A new nucleoside analog, 9-[[2-hydroxy-1-(hydroxymethyl)ethoxyl]methyl]guanine, highly active in vitro against herpes simplex virus types 1 and 2.一种新的核苷类似物，9-[[2-羟基-1-(羟甲基)乙氧基]甲基]鸟嘌呤，在体外对1型和2型单纯疱疹病毒具有高度活性。
Antimicrob Agents Chemother. 1982 Jul;22(1):55-61. doi: 10.1128/AAC.22.1.55.
7
Transcriptional control signals of a eukaryotic protein-coding gene.真核生物蛋白质编码基因的转录控制信号
Science. 1982 Jul 23;217(4557):316-24. doi: 10.1126/science.6283634.
8
Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters.单纯疱疹病毒α基因的调控：由胸苷激酶与α基因启动子融合产生的嵌合基因的表达。
Cell. 1981 May;24(2):555-65. doi: 10.1016/0092-8674(81)90346-9.
9
Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.单纯疱疹病毒1型胸苷激酶基因的核苷酸序列
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1441-5. doi: 10.1073/pnas.78.3.1441.
10
Herpes simplex virus thymidine kinase transcripts are absent from both nucleus and cytoplasm during infection in the presence of cycloheximide.在存在环己酰亚胺的情况下进行感染时，单纯疱疹病毒胸苷激酶转录本在细胞核和细胞质中均不存在。
J Virol. 1980 Nov;36(2):361-5. doi: 10.1128/JVI.36.2.361-365.1980.