来自大肠杆菌的YeeO输出黄素。
YeeO from Escherichia coli exports flavins.
作者信息
McAnulty Michael J, Wood Thomas K
机构信息
a Department of Chemical Engineering ; Pennsylvania State University; University Park , PA USA.
出版信息
Bioengineered. 2014;5(6):386-92. doi: 10.4161/21655979.2014.969173. Epub 2014 Nov 11.
Abstract
Multidrug and toxic compound extrusion (MATE) proteins help maintain cellular homeostasis by secreting metabolic wastes. Flavins may occur as cellular waste products, with their production and secretion providing potential benefit for industrial applications related to biofuel cells. Here we find that MATE protein YeeO from Escherichia coli exports both flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Significant amounts of flavins were trapped intracellularly when YeeO was produced indicating transport limits secretion of flavins. Wild-type E. coli secreted 3 flavins (riboflavin, FMN, and FAD), so E. coli likely produces additional flavin transporters.
摘要
多药及有毒化合物外排（MATE）蛋白通过分泌代谢废物来帮助维持细胞内稳态。黄素可能作为细胞废物产生，其产生和分泌为与生物燃料电池相关的工业应用提供了潜在益处。在此，我们发现来自大肠杆菌的MATE蛋白YeeO可输出黄素单核苷酸（FMN）和黄素腺嘌呤二核苷酸（FAD）。当产生YeeO时，大量黄素被困在细胞内，这表明转运限制了黄素的分泌。野生型大肠杆菌分泌3种黄素（核黄素、FMN和FAD），因此大肠杆菌可能还产生了其他黄素转运蛋白。
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