MVZ for Histology, Cytology and Molecular Diagnostics, Trier, Germany.
Institute for Pathology, University of Heidelberg, Heidelberg, Germany.
Int J Oncol. 2015 Mar;46(3):893-906. doi: 10.3892/ijo.2014.2788. Epub 2014 Dec 4.
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) imaging mass spectrometry (IMS) is an evolving technique in cancer diagnostics and combines the advantages of mass spectrometry (proteomics), detection of numerous molecules, and spatial resolution in histological tissue sections and cytological preparations. This method allows the detection of proteins, peptides, lipids, carbohydrates or glycoconjugates and small molecules.Formalin-fixed paraffin-embedded tissue can also be investigated by IMS, thus, this method seems to be an ideal tool for cancer diagnostics and biomarker discovery. It may add information to the identification of tumor margins and tumor heterogeneity. The technique allows tumor typing, especially identification of the tumor of origin in metastatic tissue, as well as grading and may provide prognostic information. IMS is a valuable method for the identification of biomarkers and can complement histology, immunohistology and molecular pathology in various fields of histopathological diagnostics, especially with regard to identification and grading of tumors.
基质辅助激光解吸/电离（MALDI）飞行时间（TOF）成像质谱（IMS）是癌症诊断中的一项不断发展的技术，它结合了质谱（蛋白质组学）的优势、对组织切片和细胞学制剂中众多分子的检测以及空间分辨率。该方法可检测蛋白质、肽、脂质、碳水化合物或糖缀合物以及小分子。通过 IMS 也可以研究福尔马林固定石蜡包埋组织，因此，该方法似乎是癌症诊断和生物标志物发现的理想工具。它可以为肿瘤边缘和肿瘤异质性的鉴定提供信息。该技术可以进行肿瘤分型，特别是在转移组织中鉴定肿瘤起源，还可以进行分级，并提供预后信息。IMS 是鉴定生物标志物的有价值的方法，可以补充组织学、免疫组织化学和分子病理学在组织病理学诊断的各个领域中的应用，特别是在肿瘤的鉴定和分级方面。
