Roiban Gheorghe-Doru, Reetz Manfred T
GlaxoSmithKline R&D Ltd, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, UK.
Chem Commun (Camb). 2015 Feb 11;51(12):2208-24. doi: 10.1039/c4cc09218j.
Cytochrome P450 enzymes (CYPs) have been used for more than six decades as catalysts for the CH-activating oxidative hydroxylation of organic compounds with formation of added-value products. However, it has not been possible to control regio- and stereoselectivity in a general manner, which is necessary for wide (industrial) applications. Especially directed evolution as a Darwinian approach to protein engineering has changed the situation for the better during the last 3-4 years leading to extensive progress, which is summarized and analysed in this Feature Article.
细胞色素P450酶（CYPs）作为有机化合物CH-活化氧化羟基化反应的催化剂已被使用了六十多年，可生成增值产品。然而，尚未能够以通用方式控制区域选择性和立体选择性，而这对于广泛的（工业）应用来说是必需的。特别是定向进化作为一种蛋白质工程的达尔文方法，在过去3至4年中使情况得到了改善，取得了广泛的进展，本专题文章对此进行了总结和分析。
