Kolberg Carolina, Horst Andréa, Moraes Maira S, Duarte Felipe Coutinho Kullmann, Riffel Ana Paula Konzen, Scheid Taína, Kolberg Angela, Partata Wania A
Academic Researcher, Department of Physiology, Institute of Basic Health Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Academic Researcher, Department of Physiology, Institute of Basic Health Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
J Manipulative Physiol Ther. 2015 Feb;38(2):119-29. doi: 10.1016/j.jmpt.2014.11.003. Epub 2014 Dec 5.
The purpose of this study was to investigate oxidative-stress parameters in individuals with chronic neck or back pain after 5 weeks of treatment with high-velocity, low-amplitude (HVLA) spinal manipulation.
Twenty-three individuals aged 38.2 ± 11.7 years with nonspecific chronic neck or back pain verified by the Brazilian Portuguese version of the Chronic Pain Grade, with a sedentary lifestyle, no comorbidities, and not in adjuvant therapy, underwent treatment with HVLA chiropractic manipulation twice weekly for 5 weeks. Therapeutic procedures were carried out by an experienced chiropractor. Blood samples were assessed before and after treatment to determine the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx), and the levels of nitric oxide metabolites and lipid hydroperoxides. These blood markers were analyzed by paired Student t test. Differences were considered statistically significant, when P was <.05.
There was no change in catalase but an increase in SOD (0.35 ± 0.03 U SOD per milligram of protein vs 0.44 ± 0.04 U SOD per milligram of protein; P < .05) and GPx (7.91 ± 0.61 nmol/min per milligram of protein vs 14.07 ± 1.07 nmol/min per milligram of protein; P < .001) activities after the treatment. The nitric oxide metabolites and the lipid hydroperoxides did not change after treatment.
High-velocity, low-amplitude spinal manipulation twice weekly for 5 weeks increases the SOD and GPx activities. Previous studies have shown a relationship between pain and oxidative and nitrosative parameters; thus, it is possible that changes in these enzymes might be related to the analgesic effect of HVLA spinal manipulation.
本研究旨在调查经高速低幅（HVLA）脊柱手法治疗5周后慢性颈痛或背痛患者的氧化应激参数。
23名年龄为38.2±11.7岁的个体，经巴西葡萄牙语版慢性疼痛分级验证为非特异性慢性颈痛或背痛，久坐不动，无合并症，未接受辅助治疗，接受HVLA脊椎按摩治疗，每周两次，共5周。治疗程序由一名经验丰富的脊椎按摩师进行。在治疗前后采集血样，以测定抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GPx）的活性，以及一氧化氮代谢物和脂质氢过氧化物的水平。这些血液标志物通过配对学生t检验进行分析。当P<0.05时，差异被认为具有统计学意义。
过氧化氢酶无变化，但治疗后SOD活性增加（每毫克蛋白质0.35±0.03 U SOD vs每毫克蛋白质0.44±0.04 U SOD；P<0.05），GPx活性增加（每毫克蛋白质7.91±0.61 nmol/min vs每毫克蛋白质14.07±1.07 nmol/min；P<0.001）。治疗后一氧化氮代谢物和脂质氢过氧化物没有变化。
每周两次、持续5周的高速低幅脊柱手法治疗可增加SOD和GPx活性。先前的研究表明疼痛与氧化和亚硝化参数之间存在关联；因此，这些酶的变化可能与HVLA脊柱手法治疗的镇痛效果有关。
