Wagner Vivian Petersen, Martins Manoela Domingues, Dillenburg Caroline Siviero, Meurer Luise, Castilho Rogerio Moraes, Squarize Cristiane Helena
Department of Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil 90035.
Department of Pathology, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil 90035.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2015 Mar;119(3):310-7. doi: 10.1016/j.oooo.2014.10.006. Epub 2014 Oct 18.
Keratoacanthoma (KA), a keratinocytic neoplasm, is associated with sun exposure and is often found in the head and neck area, including the lip. KA is thought to arise from hair follicle cells, but its origin is largely unknown. Keratins (Ks) and histochemical stains are of great value to characterize and identify normal and neoplastic cells. The objective of this study is to analyze a panel of Ks and periodic acid-Schiff (PAS) staining on KA.
Using KA biopsies from the lips and normal skin samples, we performed immunohistochemical and histochemical profiling to determine which biomarkers are conserved between tumors and normal tissues.
The normal hair follicle has multiple well-defined compartments. The outer root sheath (ORS) cells presented K6 and K14 and were also PAS positive. In addition, the infundibulum cells showed positive labeling to K10. Hair cortex keratin was observed in the cortical and precortical cells. Interestingly, KA tumor cells were positive for PAS, K6, K10, and K14 but not to hair cortex keratin. Lip and skin epithelium were negative for PAS and K6.
Our results indicate that KA of the lip is derived from ORS cells, particularly those cells associated with the upper ORS.
角化棘皮瘤（KA）是一种角质形成细胞肿瘤，与阳光照射有关，常发生于头颈部区域，包括唇部。KA被认为起源于毛囊细胞，但其起源在很大程度上尚不清楚。角蛋白（Ks）和组织化学染色对于表征和识别正常及肿瘤细胞具有重要价值。本研究的目的是分析一组角蛋白和过碘酸希夫（PAS）染色在KA中的情况。
我们使用唇部KA活检组织和正常皮肤样本，进行免疫组织化学和组织化学分析，以确定肿瘤组织和正常组织之间哪些生物标志物是保守的。
正常毛囊有多个界限分明的区域。外根鞘（ORS）细胞表达K6和K14，且PAS染色也呈阳性。此外，漏斗部细胞对K10呈阳性标记。在皮质和前皮质细胞中观察到毛发皮质角蛋白。有趣的是，KA肿瘤细胞PAS、K6、K10和K14呈阳性，但对毛发皮质角蛋白呈阴性。唇部和皮肤上皮PAS和K6呈阴性。
我们的结果表明，唇部KA起源于ORS细胞，特别是那些与上ORS相关的细胞。
