巴基斯坦家禽饲料及饲料原料中赭曲霉毒素A的存在情况。
Occurrence of ochratoxin A in poultry feeds and feed ingredients from Pakistan.
作者信息
Sherazi S T H, Shar Z H, Sumbal G A, Tan Eddie T, Bhanger M I, Kara Huseyin, Nizamani S M
机构信息
National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
出版信息
Mycotoxin Res. 2015 Feb;31(1):1-7. doi: 10.1007/s12550-014-0216-0. Epub 2014 Dec 10.
A study was conducted to evaluate the occurrence of ochratoxin A (OTA) in complete poultry feeds (n=80) and poultry feed ingredients (n=286) from Pakistan. All samples were first analyzed by indirect enzyme linked immunosorbent assay (ELISA), samples with OTA concentrations above the European Union maximum regulatory limit (MRL, 100 μg/kg) were further confirmed by HPLC-FLD. Contamination frequency and mean OTA levels were 31% and 51 μg/kg in feed ingredients, and the corresponding values for complete feeds were 38% and 75 μg/kg. Ten samples of complete poultry feed and 19 samples of feed ingredients contained OTA at levels higher than the MRL. The results of the present study indicate that there is a strong need for a more intense monitoring programs for OTA in poultry feed.
开展了一项研究，以评估来自巴基斯坦的全价家禽饲料（n = 80）和家禽饲料原料（n = 286）中赭曲霉毒素A（OTA）的存在情况。所有样品首先通过间接酶联免疫吸附测定（ELISA）进行分析，OTA浓度高于欧盟最大监管限量（MRL，100μg/kg）的样品通过高效液相色谱-荧光检测法（HPLC-FLD）进一步确认。饲料原料中的污染频率和OTA平均水平分别为31%和51μg/kg，全价饲料的相应值分别为38%和75μg/kg。10份全价家禽饲料样品和19份饲料原料样品中的OTA含量高于MRL。本研究结果表明，迫切需要对家禽饲料中的OTA开展更密集的监测计划。
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