意大利西北部有机和标准养猪场饲料和血清中赭曲霉毒素 A 污染的调查。

A survey of ochratoxin A contamination in feeds and sera from organic and standard swine farms in northwest Italy.

机构信息

Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università di Torino, Grugliasco, Turin, Italy.

出版信息

J Sci Food Agric. 2010 Jul;90(9):1467-72. doi: 10.1002/jsfa.3965.

DOI:10.1002/jsfa.3965

PMID:20549798

Abstract

BACKGROUND

A survey was carried out on conventional (n = 11) and organic (n = 4) swine farms in northwest Italy in order to investigate the occurrence of ochratoxin A (OTA) in feed and serum samples collected from September 2006 to March 2009. Each farm was sampled twice and a total of 30 feed samples and 285 serum samples were collected. OTA levels were determined through extraction, immunoaffinity column purification and high-performance liquid chromatography analysis coupled with fluorimetric detection.

RESULTS

All feed samples resulted to be contaminated with OTA at levels ranging from 0.22 to 38.4 microg kg(-1). The OTA concentrations found in organic feed samples were significantly higher (P < 0.05) than those found in conventional feed samples. All serum samples resulted to be contaminated with OTA at levels ranging from 0.03 to 6.24 ng mL(-1). The OTA concentrations found in organic serum samples were significantly higher (P < 0.001) than those found in conventional serum samples.

CONCLUSION

None of the feed samples contained more than the maximum level (50 microg OTA kg(-1), considering a feed moisture content of 120 g kg(-1)) recommended by the European Commission for OTA in complementary and complete swine feedstuffs. The OTA contamination of organic feed and serum samples was found to be significantly higher than that of conventional feed and serum samples.

摘要

背景

为了调查饲料和血清样本中赭曲霉毒素 A（OTA）的发生情况，我们在意大利西北部对常规（n=11）和有机（n=4）养猪场进行了一项调查。从 2006 年 9 月到 2009 年 3 月，每个农场进行了两次采样，共采集了 30 个饲料样本和 285 个血清样本。通过提取、免疫亲和柱纯化和高效液相色谱分析结合荧光检测来确定 OTA 水平。

结果

所有饲料样本均被 OTA 污染，浓度范围为 0.22 至 38.4μg/kg。有机饲料样本中发现的 OTA 浓度明显高于（P<0.05）常规饲料样本。所有血清样本均被 OTA 污染，浓度范围为 0.03 至 6.24ng/mL。有机血清样本中发现的 OTA 浓度明显高于（P<0.001）常规血清样本。