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DNA topoisomerase II activity in nonreplicating, transcriptionally inactive, chicken late spermatids.

作者信息

Roca J, Mezquita C

机构信息

Department of Physiological Sciences, Faculty of Medicine, University of Barcelona, Spain.

出版信息

EMBO J. 1989 Jun;8(6):1855-60. doi: 10.1002/j.1460-2075.1989.tb03581.x.

DOI:10.1002/j.1460-2075.1989.tb03581.x
PMID:2548858
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC401034/
Abstract

To study a possible differential involvement of type I and type II DNA topoisomerases in the functional and structural changes that chromatin undergoes during spermatogenesis, we have determined both enzymatic activities in chicken testis cell nuclei at successive stages of differentiation. Whereas DNA topoisomerase I varies in parallel with transcriptional activity, DNA topoisomerase II was present in both replicating, transcriptionally active chicken testis cells and nonreplicating, transcriptionally inactive late spermatids. The presence of DNA topoisomerase II activity in late spermatids and, in addition, the relative increment of drug-induced topo-II-mediated DNA cleavage detected in these cells, suggest that DNA topoisomerase II might modulate the topology of DNA during the marked changes that chromatin structure undergoes in the nucleohistone-nucleoprotamine transition at the end of the spermiogenesis and could be involved in the final organization of DNA within the nucleus of the male gamete.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deef/401034/8b7f44a87003/emboj00130-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deef/401034/e9030578ff7a/emboj00130-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deef/401034/4ddfedcbfca7/emboj00130-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deef/401034/8b7f44a87003/emboj00130-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deef/401034/e9030578ff7a/emboj00130-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deef/401034/4ddfedcbfca7/emboj00130-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deef/401034/8b7f44a87003/emboj00130-0220-a.jpg

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1
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本文引用的文献

1
The organisation of chromatin loops: characterization of a scaffold attachment site.染色质环的组织：支架附着位点的特征化。
EMBO J. 1986 Mar;5(3):511-8. doi: 10.1002/j.1460-2075.1986.tb04240.x.
2
Mitotic spindle pulls but fails to separate chromosomes in type II DNA topoisomerase mutants: uncoordinated mitosis.有丝分裂纺锤体在II型DNA拓扑异构酶突变体中牵引但未能分离染色体：有丝分裂不协调。
EMBO J. 1986 May;5(5):1003-10. doi: 10.1002/j.1460-2075.1986.tb04315.x.
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Genotoxic effects of ethyl methanesulfonate and X-rays at different stages of rat spermatogenesis, studied by inhibition of DNA synthesis and induction of DNA repair in vitro.
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Topoisomerase II regulates yeast genes with singular chromatin architectures.拓扑异构酶 II 调控具有单一染色质结构的酵母基因。
Nucleic Acids Res. 2013 Nov;41(20):9243-56. doi: 10.1093/nar/gkt707. Epub 2013 Aug 9.
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Regulating DNA supercoiling: sperm points the way.调控 DNA 超螺旋结构：精子指引方向。
Biol Reprod. 2011 May;84(5):841-3. doi: 10.1095/biolreprod.111.090951. Epub 2011 Jan 19.
6
Poly(ADP-ribose) polymerases PARP1 and PARP2 modulate topoisomerase II beta (TOP2B) function during chromatin condensation in mouse spermiogenesis.多聚（ADP-核糖）聚合酶 PARP1 和 PARP2 在小鼠精子发生过程中的染色质浓缩过程中调节拓扑异构酶 IIβ（TOP2B）的功能。
Biol Reprod. 2011 May;84(5):900-9. doi: 10.1095/biolreprod.110.090035. Epub 2011 Jan 12.
7
Topoisomerase II, not topoisomerase I, is the proficient relaxase of nucleosomal DNA.拓扑异构酶II而非拓扑异构酶I是核小体DNA的有效解旋酶。
EMBO J. 2006 Jun 7;25(11):2575-83. doi: 10.1038/sj.emboj.7601142. Epub 2006 May 18.
8
Contribution of topoisomerase I to conversion of single-strand into double-strand DNA breaks.拓扑异构酶I在单链DNA断裂转化为双链DNA断裂过程中的作用。
Mol Biol Rep. 1998 Jan;25(1):21-6. doi: 10.1023/a:1006831527609.
9
The testis-specific high-mobility-group protein, a phosphorylation-dependent DNA-packaging factor of elongating and condensing spermatids.睾丸特异性高迁移率族蛋白，一种参与延长和浓缩精子细胞的磷酸化依赖性DNA包装因子。
Mol Cell Biol. 1996 Jul;16(7):3720-9. doi: 10.1128/MCB.16.7.3720.
10
Histone hyperacetylation can induce unfolding of the nucleosome core particle.组蛋白高度乙酰化可诱导核小体核心颗粒解折叠。
Nucleic Acids Res. 1990 May 11;18(9):2739-47. doi: 10.1093/nar/18.9.2739.
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DNA density in mitotic and meiotic metaphase chromosomes of plants and animals.动植物有丝分裂和减数分裂中期染色体中的DNA密度。
J Cell Sci. 1983 Sep;63:173-9. doi: 10.1242/jcs.63.1.173.
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DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.酿酒酵母的DNA拓扑异构酶II突变体：在DNA复制终止时，拓扑异构酶II对于子分子的分离是必需的。
Proc Natl Acad Sci U S A. 1984 May;81(9):2616-20. doi: 10.1073/pnas.81.9.2616.
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DNA topoisomerases from rat liver: physiological variations.大鼠肝脏中的DNA拓扑异构酶：生理变异
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Novel topologically knotted DNA from bacteriophage P4 capsids: studies with DNA topoisomerases.来自噬菌体P4衣壳的新型拓扑打结DNA：DNA拓扑异构酶研究
Nucleic Acids Res. 1981 Aug 25;9(16):3979-89. doi: 10.1093/nar/9.16.3979.
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DNA topoisomerases.DNA拓扑异构酶
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DNA topoisomerases.DNA拓扑异构酶
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