Mlalila Nichrous, Swai Hulda, Kalombo Lonji, Hilonga Askwar
School of Life Sciences and Bioengineering, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.
Materials Science and Manufacturing, Council for Scientific and Industrial Research, Pretoria, South Africa.
Nanotechnol Sci Appl. 2014 Dec 1;7:105-12. doi: 10.2147/NSA.S72083. eCollection 2014.
The goal of this study was to explore the effects of spray-drying on w/o/w double emulsions of methyltestosterone (MT) loaded in a stearic acid matrix. MT-loaded nanoparticles were formulated by a water-in-oil-in-water emulsion technique using 50, 75, and 100 mg of stearic acid, 2% and 3% w/v polyvinyl alcohol, 5% w/v lactose, and 0.2% w/v chitosan. The emulsions were immediately spray-dried based on an optimized model of inlet temperature and pump rate, and characterized for optimized responses with regard to particle size, polydispersity index, and zeta potential, for both emulsion and powder samples. Dynamic light scattering analysis shown that the nanoparticles increased in size with increasing concentrations of polyvinyl alcohol and stearic acid. Scanning electron microscopy indicated that the MT-loaded nanoparticles were spherical in shape, had a smooth surface, and were in an amorphous state, which was confirmed by differential scanning calorimetry. These MT-loaded nanoparticles are a promising candidate carrier for the delivery of MT; however, further studies are needed in order to establish the stability of the system and the cargo release profile under normal conditions of use.
本研究的目的是探讨喷雾干燥对载于硬脂酸基质中的甲基睾酮（MT）的水包油包水双重乳液的影响。采用水包油包水乳液技术，使用50、75和100mg硬脂酸、2%和3%（w/v）聚乙烯醇、5%（w/v）乳糖和0.2%（w/v）壳聚糖制备了载MT的纳米颗粒。基于优化的进样温度和泵速模型，对乳液立即进行喷雾干燥，并对乳液和粉末样品的粒径、多分散指数和zeta电位等优化响应进行表征。动态光散射分析表明，纳米颗粒的尺寸随着聚乙烯醇和硬脂酸浓度的增加而增大。扫描电子显微镜表明，载MT的纳米颗粒呈球形，表面光滑，呈无定形状态，差示扫描量热法证实了这一点。这些载MT的纳米颗粒是MT递送的有前景的候选载体；然而，为了确定该系统在正常使用条件下的稳定性和货物释放情况，还需要进一步研究。
