Berardi Anna C, Oliva Francesco, Berardocco Martina, la Rovere Marina, Accorsi Patrizia, Maffulli Nicola
UOC Immunohematology and Transfusion Medicine Laboratories, Laboratory of Stem Cells, Spirito Santo Hospital, Pescara, Italy.
Department of Orthopedics and Traumatology, University of Rome "Tor Vergata", School of Medicine, Rome, Italy.
Muscles Ligaments Tendons J. 2014 Nov 17;4(3):285-91. eCollection 2014 Jul.
we previously demonstrated the presence of high levels of thyroid hormones (THs) receptors isoforms in healthy tendons, their protective action during tenocyte apoptosis, and the capability to enhance tenocyte proliferation in vitro. In the present study we tested the ability of THs to influence ECM protein tenocyte secretion in an in vitro system.
primary tenocyte-like cells were cultivated for 1, 7 and 14 days in the presence of T3 or T4 individually or in combination with ascorbic acid (AA).
THs (T3 or T4) in synergism with AA increase significantly the total collagen production after 14 days. THs in synergism with AA increase significantly the expression of collagen I,biglycan and COMP, after some days.
THs play a role on the extra cellular matrix of tendons, enhancing in vitro the production of several proteins such as collagen I, biglycan and COMP. THs receptors are active on human tenocytes, and can play a role in tendon ailments.
我们之前证明了健康肌腱中存在高水平的甲状腺激素（THs）受体亚型，它们在肌腱细胞凋亡过程中具有保护作用，并且能够在体外增强肌腱细胞增殖。在本研究中，我们测试了THs在体外系统中影响细胞外基质（ECM）蛋白肌腱细胞分泌的能力。
原代肌腱样细胞在单独存在T3或T4或与抗坏血酸（AA）联合的情况下培养1、7和14天。
14天后，THs（T3或T4）与AA协同作用显著增加总胶原蛋白产量。几天后，THs与AA协同作用显著增加I型胶原蛋白、双糖链蛋白聚糖和富含半胱氨酸的蛋白聚糖（COMP）的表达。
THs在肌腱的细胞外基质中发挥作用，在体外增强几种蛋白质如I型胶原蛋白、双糖链蛋白聚糖和COMP的产生。THs受体在人肌腱细胞上具有活性，并可在肌腱疾病中发挥作用。
