大肠杆菌rho+和rho-15菌株中DNA拓扑异构酶、单链DNA结合蛋白及DNA聚合酶I的水平。
Levels of DNA topoisomerases, single-stranded-DNA-binding protein, and DNA polymerase I in rho+ and rho-15 strains of Escherichia coli.
作者信息
Arnold G F, Phillips T A, Tessman I
机构信息
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
出版信息
J Bacteriol. 1989 Sep;171(9):5183-6. doi: 10.1128/jb.171.9.5183-5186.1989.
The Escherichia coli rho-15 mutant, which is highly defective in transcription termination, was examined to see whether its reduced DNA superhelicity could be explained by altered expression of proteins that may affect DNA structure. Levels of DNA gyrase and topoisomerase I were normal; levels of single-stranded-DNA-binding protein, DNA polymerase I, and a protein tentatively identified as Lon were significantly altered.
对转录终止存在高度缺陷的大肠杆菌rho - 15突变体进行了检测，以确定其DNA超螺旋性降低是否可以通过可能影响DNA结构的蛋白质表达改变来解释。DNA促旋酶和拓扑异构酶I的水平正常；单链DNA结合蛋白、DNA聚合酶I以及一种初步鉴定为Lon的蛋白质的水平发生了显著改变。
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