大肠杆菌rep和rho基因的相对图谱定位。
Relative map location of the rep and rho genes of Escherichia coli.
作者信息
Tessman I, Fassler J S, Bennett D C
出版信息
J Bacteriol. 1982 Sep;151(3):1637-40. doi: 10.1128/jb.151.3.1637-1640.1982.
Abstract
The rep gene of Escherichia coli was mapped between ilvC and rho by three-factor P1 transductional crosses and also by complementation with a set of lambda transducing phages that contain known amounts of bacterial DNA linked to ilvC. The physical distance between ilvC and rep and between rep and rho were calculated with an accuracy of +/- 0.4 kilobase to be 0 less than or equal to ilvC-rep less than or equal to 3.4 kilobases and 2.0 less than or equal to rep-rho less than or equal to 6.0 kilobases. It was shown that rho-15 is Gro+ for phage ST-1. An ilv::Tn10 mutation was located in ilvY.
摘要
通过三因子P1转导杂交以及与一组含有与ilvC相连的已知量细菌DNA的λ转导噬菌体进行互补，将大肠杆菌的rep基因定位在ilvC和rho之间。计算出ilvC与rep之间以及rep与rho之间的物理距离，精度为±0.4千碱基，结果为0≤ilvC - rep≤3.4千碱基，2.0≤rep - rho≤6.0千碱基。结果表明，rho - 15对噬菌体ST - 1呈Gro+。一个ilv::Tn10突变位于ilvY中。
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