van Nieuwstadt A P, Zetstra T, Boonstra J
Central Veterinary Institute, Department of Virology, Lelystad, The Netherlands.
Vet Rec. 1989 Jul 15;125(3):58-60. doi: 10.1136/vr.125.3.58.
Eight nine-week-old specific-pathogen-free pigs which had been infected with the transmissible gastroenteritis virus (TGEV)-related porcine respiratory coronavirus (PRCV) and four uninfected littermates were challenged with TGEV. The previous PRCV infection failed to protect them against the enteric TGEV infection. Virus excretion in faeces was detected by an ELISA in all the pigs for three to six consecutive days after inoculation. Although little diarrhoea was observed, the infection extended through much of the small intestine of one of the previously infected pigs four days after inoculation. Challenge with TGEV caused a secondary neutralising antibody response. By using a peroxidase conjugate of a monoclonal antibody which recognises a specific antigenic site on TGEV, antibodies against TGEV could be distinguished from antibodies against PRCV in an ELISA blocking test.
八只九周龄的无特定病原体猪，已感染与传染性胃肠炎病毒（TGEV）相关的猪呼吸道冠状病毒（PRCV），还有四只未感染的同窝仔猪，用TGEV进行攻毒。先前的PRCV感染未能保护它们免受肠道TGEV感染。接种后，通过ELISA在所有猪中连续三到六天检测粪便中的病毒排泄情况。尽管观察到很少的腹泻，但在接种四天后，其中一只先前感染的猪的大部分小肠都出现了感染。用TGEV攻毒引发了二次中和抗体反应。通过使用识别TGEV上特定抗原位点的单克隆抗体的过氧化物酶偶联物，在ELISA阻断试验中可以将抗TGEV抗体与抗PRCV抗体区分开来。
