抗瘟病毒单克隆抗体的比较分析：国际研讨会报告

Comparative analysis of monoclonal antibodies against pestiviruses: report of an international workshop.

作者信息

Cay B, Chappuis G, Coulibaly C, Dinter Z, Edwards S, Greiser-Wilke I, Gunn M, Have P, Hess G, Juntti N

机构信息

Institut National de Recherches Vétérinaires, Bruxelles, Belgium.

出版信息

Vet Microbiol. 1989 Jun;20(2):123-9. doi: 10.1016/0378-1135(89)90035-7.

DOI:10.1016/0378-1135(89)90035-7

PMID:2549680

Abstract

Thirty-three pestivirus strains were grown in cell culture and characterized by immunostaining with 19 monoclonal antibodies (MAbs) raised against hog cholera virus (HCV), with 42 MAbs against bovine viral diarrhoea virus (BVDV) and with 13 MAbs against border disease virus (BDV). Seven MAbs reacted with all pestivirus strains tested, eight MAbs detected only the seven HCV strains, three detected only the 16 BVDV strains. No MAb was found that was specific for BDV. BVDV and BDV strains were broadly cross-reactive with the MAbs, indicating a close relationship between these two species, whereas HCV strains were characterized as distinct from BVDV and BDV.

摘要

33株瘟病毒在细胞培养中生长，并通过用19种针对猪瘟病毒（HCV）产生的单克隆抗体（MAb）、42种针对牛病毒性腹泻病毒（BVDV）的MAb和13种针对边界病病毒（BDV）的MAb进行免疫染色来鉴定。7种MAb与所有测试的瘟病毒株反应，8种MAb仅检测到7株HCV株，3种仅检测到16株BVDV株。未发现对BDV具有特异性的MAb。BVDV和BDV株与MAb广泛交叉反应，表明这两个病毒种之间关系密切，而HCV株被鉴定为与BVDV和BDV不同。