Hakogi E, Tamura H, Tanaka S, Kohata A, Shimada Y, Tabuchi K
Sumoto Livestock Hygiene Service Center, Hyogo-ken, Japan.
Vet Microbiol. 1989 Jul;20(3):267-74. doi: 10.1016/0378-1135(89)90050-3.
A chromogenic limulus test ("Toxicolor") was applied to cow's milk and plasma after treatment with perchloric acid to remove interfering factors. The endotoxin levels in normal cow's milk and plasma were all less than 10 pg ml-1. In acute mastitis, the milk endotoxin level averaged (1.1 +/- 0.7) X 10(3) pg ml-1 in the cases where Gram-negative bacteria were isolated, while the plasma endotoxin concentration was normal. The endotoxin levels in the quarters infected with Gram-positive bacteria were all normal, both in milk and plasma. In gangrenous mastitis due to Gram-negative bacteria, the endotoxin concentration was very high in both milk [(9.3 +/- 5.3) X 10(6) pg ml-1] and plasma (85.2 +/- 68.2 pg ml-1). In similar cases due to Gram-positive bacteria, endotoxin levels were all normal, both in milk and plasma, resembling the acute mastitis due to Gram-positive bacteria. The test was considered suitable for the diagnosis of mastitis due to Gram-negative organisms and the levels of endotoxin detected would aid in assessing the prognosis.
用高氯酸处理牛奶和血浆以去除干扰因素后，应用显色鲎试剂法（“Toxicolor”）进行检测。正常牛奶和血浆中的内毒素水平均低于10 pg/ml。在急性乳腺炎中，分离出革兰氏阴性菌的病例中，牛奶内毒素水平平均为(1.1±0.7)×10³ pg/ml，而血浆内毒素浓度正常。感染革兰氏阳性菌的乳腺象限中，牛奶和血浆中的内毒素水平均正常。在革兰氏阴性菌引起的坏疽性乳腺炎中，牛奶[(9.3±5.3)×10⁶ pg/ml]和血浆(85.2±68.2 pg/ml)中的内毒素浓度都非常高。在革兰氏阳性菌引起的类似病例中，牛奶和血浆中的内毒素水平均正常，类似于革兰氏阳性菌引起的急性乳腺炎。该检测被认为适用于诊断革兰氏阴性菌引起的乳腺炎，检测到的内毒素水平有助于评估预后。
