Haffer K, Gustafson D P, Kanitz C L
J Clin Microbiol. 1980 Mar;11(3):217-9. doi: 10.1128/jcm.11.3.217-219.1980.
An indirect hemagglutination test for the detection of antibodies in swine serum specific for pseudorabies virus is described. The indirect hemagglutination test was less time consuming than the standardized virus nertralization test while being highly sensitive and accurate. Serum samples that were toxic to virus neutralization indicator cells were readily tested in the indirect hemagglutination test. The indirect hemagglutination test may also be more sensitive than the virus neutralization test for determining early titers in pseudorabies virus infections. Complete methodology is described.
本文描述了一种用于检测猪血清中针对伪狂犬病病毒特异性抗体的间接血凝试验。间接血凝试验比标准化的病毒中和试验耗时更少,同时具有高度的敏感性和准确性。对病毒中和指示细胞有毒性的血清样本在间接血凝试验中易于检测。在确定伪狂犬病病毒感染的早期滴度方面,间接血凝试验可能也比病毒中和试验更敏感。文中描述了完整的方法。