University of Granada, Faculty of Dentistry, Dental Materials Section, Spain.
Dent Mater. 2013 Aug;29(8):e142-52. doi: 10.1016/j.dental.2013.04.024. Epub 2013 Jun 10.
The objective of the study was to determine if zinc-doped etch-and-rinse dentin adhesive may induce therapeutic effects within the resin-dentin interface.
Human acid-etched dentin was infiltrated with Adper™ Single Bond Plus (SB, 3M ESPE, St. Paul, MN, USA), SB doped with 10wt.% ZnO nanoparticles (ZnO-SB) or SB doped with 2wt.% ZnCl2 (ZnCl2-SB). AFM/nanoindentation analysis was performed on fully hydrated specimens to evaluate the nanomechanical properties (Hi: hardness; Ei: modulus of elasticity) across the resin-dentin interface after different SBF storage periods (24h, 1m, 3m). Confocal laser microscopy (CLSM) was used to evaluate the ultramorphology and micropermeability at 24h and 3m of SBF storage.
SB control specimens exhibited a decrease in Hi in the hybrid layer (HL) and bottom of the hybrid layer (BHL) and a decrease in Ei in the HL after 3m of SBF storage, indicating that severe degradation occurred in the control interface. ZnO-SB bonded specimens preserved the initial Hi and Ei at the HL and BHL subsequent SBF storage; ZnCl2-SB bonded specimens showed a decrease in Ei, in the HL over time. CLSM analysis confirmed that both Zn-doped adhesives were able to preserve the integrity of the HL.
Specific formulation of Zn-doped etch-and-rinse adhesives may offer the possibility to maintain the nano-mechanical properties along the dentin-bonded interface by inhibiting dentin MMPs and by protective mineral crystals formation within the resin-dentin interface. Clinical advantages may be expected by preserving and improving the integrity of the hybrid layer when Zn-doped adhesives are employed.
本研究旨在探讨锌掺杂酸蚀冲洗型粘结剂是否能在树脂-牙本质界面产生治疗效果。
采用 Adper™SingleBond Plus（SB，3M ESPE，明尼苏达州圣保罗）、掺杂 10wt.%氧化锌纳米颗粒的 SB（ZnO-SB）或掺杂 2wt.%ZnCl2 的 SB（ZnCl2-SB）对酸蚀牙本质进行浸润。采用原子力显微镜/纳米压痕分析对完全水合的标本进行分析，以评估不同 SBF 储存时间（24h、1m、3m）后树脂-牙本质界面的纳米力学性能（Hi：硬度；Ei：弹性模量）。共聚焦激光显微镜（CLSM）用于评估 24h 和 3m SBF 储存后的超微形态和微通透性。
SB 对照组标本在 SBF 储存 3m 后，混合层（HL）和混合层底部（BHL）的 Hi 降低，HL 的 Ei 降低，表明对照界面发生严重降解。经 SBF 储存后，ZnO-SB 粘结剂在 HL 和 BHL 处保持初始 Hi 和 Ei；ZnCl2-SB 粘结剂的 Ei 在 HL 中随时间逐渐降低。CLSM 分析证实，两种锌掺杂粘结剂均能保持 HL 的完整性。
特定配方的锌掺杂酸蚀冲洗型粘结剂通过抑制牙本质 MMPs 和在树脂-牙本质界面形成保护矿物晶体，可能具有维持牙本质粘结界面纳米力学性能的潜力。当使用锌掺杂粘结剂时，预计能保留和改善混合层的完整性，从而带来临床优势。
