Meyerhans A, Cheynier R, Albert J, Seth M, Kwok S, Sninsky J, Morfeldt-Månson L, Asjö B, Wain-Hobson S
Laboratoire de Biologie et Immunologie Moléculaires des Rétrovirus, Institut Pasteur, Paris, France.
Cell. 1989 Sep 8;58(5):901-10. doi: 10.1016/0092-8674(89)90942-2.
A genetic study has been made of the HIV tat gene from sequential HIV-1 isolates and the corresponding infected peripheral blood mononuclear cells. DNA was amplified by polymerase chain reaction (PCR) and cloned into a eukaryotic expression vector. Twenty clones were sequenced from each sample. Comparing the sequential HIV isolates, abrupt differences were seen between the major forms of each isolate. These progressive changes were not reflected at all among the in vitro samples. The fluctuation in the quasispecies in vivo may suggest a much more dynamic role for latently infected mononuclear cells. High frequencies of functionally defective tat genes were identified. Given such complexity and the evident differences between quasispecies in vivo and in vitro, the task of defining HIV infection in molecular terms will be difficult.
对来自连续的HIV-1分离株以及相应感染的外周血单核细胞中的HIV tat基因进行了遗传学研究。通过聚合酶链反应（PCR）扩增DNA，并将其克隆到真核表达载体中。每个样本对20个克隆进行测序。比较连续的HIV分离株，发现每个分离株的主要形式之间存在明显差异。这些渐进性变化在体外样本中完全没有体现。体内准种的波动可能表明潜伏感染的单核细胞具有更为动态的作用。鉴定出了高频率的功能缺陷型tat基因。鉴于这种复杂性以及体内和体外准种之间的明显差异，从分子层面定义HIV感染将是一项艰巨的任务。
