有证据表明，抗原诱导的T淋巴细胞增殖需要白细胞共同抗原。
Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation.
作者信息
Pingel J T, Thomas M L
机构信息
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110.
出版信息
Cell. 1989 Sep 22;58(6):1055-65. doi: 10.1016/0092-8674(89)90504-7.
Abstract
The leukocyte-common antigen (L-CA) is a family of large molecular weight glycoproteins uniquely expressed on the surface of all nucleated cells of hematopoietic origin. The glycoprotein consists of a heavily glycosylated exterior domain, a single membrane spanning region, and a large cytoplasmic domain that contains tyrosine phosphatase activity. To investigate the function of this family, we generated T cell clones that lacked L-CA (L-CA-). The expression of the alpha beta T cell receptor, CD3, CD4, IL-2 receptor (p55), LFA-1, Thy-1, and Pgp-1 (CD44) was normal. The L-CA- T cell clones failed to proliferate in response to antigen or cross-linked CD3; however, they could still proliferate in response to IL-2. An L-CA+ revertant was obtained and the ability to proliferate in response to antigen and cross-linked CD3 was restored. These data indicate that L-CA is required for T cells to enter into cell cycle in response to antigen.
摘要
白细胞共同抗原（L-CA）是一类大分子量糖蛋白家族，特异性表达于所有造血来源的有核细胞表面。该糖蛋白由一个高度糖基化的外部结构域、一个单次跨膜区域和一个含有酪氨酸磷酸酶活性的大细胞质结构域组成。为了研究这个家族的功能，我们构建了缺乏L-CA（L-CA-）的T细胞克隆。αβ T细胞受体、CD3、CD4、白细胞介素-2受体（p55）、淋巴细胞功能相关抗原-1（LFA-1）、Thy-1和Pgp-1（CD44）的表达均正常。L-CA- T细胞克隆在抗原或交联的CD3刺激下不能增殖；然而，它们仍能在白细胞介素-2刺激下增殖。获得了一个L-CA+回复突变体，其在抗原和交联的CD3刺激下的增殖能力得以恢复。这些数据表明，L-CA是T细胞响应抗原进入细胞周期所必需的。
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