Epis Sara, Porretta Daniele, Mastrantonio Valentina, Urbanelli Sandra, Sassera Davide, De Marco Leone, Mereghetti Valeria, Montagna Matteo, Ricci Irene, Favia Guido, Bandi Claudio
Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
Department of Environmental Biology, University "La Sapienza" of Rome, Rome, Italy.
Sci Rep. 2014 Dec 11;4:7435. doi: 10.1038/srep07435.
In insects, ABC transporters have been shown to contribute to defence/resistance to insecticides by reducing toxic concentrations in cells/tissues. Despite the extensive studies about this detoxifying mechanism, the temporal patterns of ABC transporter activation have been poorly investigated. Using the malaria vector Anopheles stephensi as a study system, we investigated the expression profile of ABC genes belonging to different subfamilies in permethrin-treated larvae at different time points (30 min to 48 h). Our results showed that the expression of ABCB and ABCG subfamily genes was upregulated at 1 h after treatment, with the highest expression observed at 6 h. Therefore, future investigations on the temporal dynamics of ABC gene expression will allow a better implementation of insecticide treatment regimens, including the use of specific inhibitors of ABC efflux pumps.
在昆虫中，ABC转运蛋白已被证明通过降低细胞/组织中的有毒浓度来促进对杀虫剂的防御/抗性。尽管对这种解毒机制进行了广泛研究，但ABC转运蛋白激活的时间模式却鲜有研究。我们以疟疾媒介斯氏按蚊作为研究系统，调查了在不同时间点（30分钟至48小时）用氯菊酯处理的幼虫中属于不同亚家族的ABC基因的表达谱。我们的结果表明，ABCB和ABCG亚家族基因的表达在处理后1小时上调，在6小时观察到最高表达。因此，未来对ABC基因表达时间动态的研究将有助于更好地实施杀虫剂治疗方案，包括使用ABC外排泵的特异性抑制剂。
