Ferrari Marco, Negri Agata, Romeo Claudia, Boccazzi Ilaria Varotto, Nodari Riccardo, Habluetzel Annette, Molteni Giorgio, Corbett Yolanda
J Am Mosq Control Assoc. 2018 Dec;34(4):311-314. doi: 10.2987/18-6779.1.
Detoxifying pathways of mosquitoes against the neem () extracts are still unclear. The aim of the present study was to investigate the role of adenosine triphosphate-binding cassette (ABC) transporters in this process in , one of the main malaria vectors in southern Asia. Third-stage larvae of were fed with fish food alone or in combination with neem extract at 0.5%, 1%, 5%, and 10%. Six ABC-transporter genes from 3 different subfamilies (B, C, and G) were analyzed to assess their relative expression compared with controls. A bioassay was also performed to assess larval mortality rate at different concentrations and in combination with verapamil, an ABC-transporter inhibitor. No significant variation in the expression levels of any transporter belonging to the B, C, and G subfamilies was detected. Furthermore, the use of verapamil did not induce an increase in mortality at any of the tested neem extract concentrations, indicating that ABC transporters are not involved in the detoxification of neem extracts in larvae.
蚊子针对印楝提取物的解毒途径仍不清楚。本研究的目的是调查三磷酸腺苷结合盒（ABC）转运蛋白在南亚主要疟疾传播媒介之一的按蚊这一过程中的作用。将按蚊的第三阶段幼虫单独喂食鱼食，或与浓度为0.5%、1%、5%和10%的印楝提取物混合喂食。分析了来自3个不同亚家族（B、C和G）的6个ABC转运蛋白基因，以评估它们与对照相比的相对表达。还进行了生物测定，以评估不同浓度以及与ABC转运蛋白抑制剂维拉帕米联合使用时的幼虫死亡率。未检测到属于B、C和G亚家族的任何转运蛋白的表达水平有显著变化。此外，在任何测试的印楝提取物浓度下，使用维拉帕米都不会导致死亡率增加，这表明ABC转运蛋白不参与按蚊幼虫对印楝提取物的解毒过程。
