Matsumoto M, Ban T, Okamoto Y
Department of Cardiovascular Surgery, Faculty of Medicine, Kyoto University, Japan.
J Cardiovasc Surg (Torino). 1989 Jul-Aug;30(4):609-13.
With scanning electron microscopy, we examined the behavior of platelets and leukocytes on the luminal surface of small caliber polyurethane grafts implanted into small arteries of dogs. Thirty-five grafts were implanted to the carotid and/or femoral arteries. The animals were treated with aspirin and/or DN9693, an inhibitor of phosphodiesterase. In the group treated with aspirin (40 mg/kg i.v.), the deposition of platelets on the luminal surface of the implanted polyurethane grafts was suppressed and the luminal surface was covered with adherent leukocytes. Fibrin nets were formed on the adherent leukocytes. In addition, the adhesion of leukocytes on the grafts was considerably suppressed in the group treated with DN9693 (50 micrograms/kg/min i.v.), and the formation of fibrin nets was markedly reduced. In contrast, in the control group the luminal surface was covered with numerous platelets and some leukocytes, which formed thrombi. These findings suggest that leukocytes adhere primarily to the prosthetic graft and play an important role in the initiation of fibrin formation.
通过扫描电子显微镜，我们检查了植入犬小动脉的小口径聚氨酯移植物管腔表面上血小板和白细胞的行为。将35个移植物植入颈动脉和/或股动脉。动物用阿司匹林和/或磷酸二酯酶抑制剂DN9693治疗。在用阿司匹林（静脉注射40mg / kg）治疗的组中，植入的聚氨酯移植物管腔表面上血小板的沉积受到抑制，管腔表面覆盖有粘附的白细胞。在粘附的白细胞上形成纤维蛋白网。此外，在用DN9693（静脉注射50μg/ kg / min）治疗的组中，白细胞在移植物上的粘附被显著抑制，并且纤维蛋白网的形成明显减少。相比之下，在对照组中，管腔表面覆盖有大量血小板和一些白细胞，它们形成血栓。这些发现表明白细胞主要粘附于人工移植物并在纤维蛋白形成的起始中起重要作用。
