Becker Johannes, Lu Lichun, Runge M Brett, Zeng Heng, Yaszemski Michael J, Dadsetan Mahrokh
Department of Orthopedic Surgery, Mayo Clinic, College of Medicine, Rochester, Minnesota, 55905.
Department of Traumatology and Sports Injuries, University Hospital Salzburg, Paracelsus Medical University Salzburg, Müllner Hauptstr, 48, Salzburg, 5020, Austria.
J Biomed Mater Res A. 2015 Aug;103(8):2549-57. doi: 10.1002/jbm.a.35391. Epub 2014 Dec 26.
In tissue engineering, development of an osteoconductive construct that integrates with host tissue remains a challenge. In this work, the effect of bone-like minerals on maturation of pre-osteoblast cells was investigated using polymer-mineral scaffolds composed of poly(propylene fumarate)-co-poly(caprolactone) (PPF-co-PCL) and nano-sized hydroxyapatite (HA). The HA of varying concentrations was added to an injectable formulation of PPF-co-PCL and the change in thermal and mechanical properties of the scaffolds was evaluated. No change in onset of degradation temperature was observed due to the addition of HA, however compressive and tensile moduli of copolymer changed significantly when HA amounts were increased in composite formulation. The change in mechanical properties of copolymer was found to correlate well to HA concentration in the constructs. Electron microscopy revealed mineral nucleation and a change in surface morphology and the presence of calcium and phosphate on surfaces was confirmed using energy dispersive X-ray analysis. To characterize the effect of mineral on attachment and maturation of pre-osteoblasts, W20-17 cells were seeded on HA/copolymer composites. We demonstrated that cells attached more to the surface of HA containing copolymers and their proliferation rate was significantly increased. Thus, these findings suggest that HA/PPF-co-PCL composite scaffolds are capable of inducing maturation of pre-osteoblasts and have the potential for use as scaffold in bone tissue engineering.
在组织工程中，开发一种能与宿主组织整合的骨传导性构建体仍然是一项挑战。在这项工作中，使用由聚（富马酸丙酯）-共-聚（己内酯）（PPF-co-PCL）和纳米级羟基磷灰石（HA）组成的聚合物-矿物质支架，研究了类骨矿物质对成骨前体细胞成熟的影响。将不同浓度的HA添加到PPF-co-PCL的可注射制剂中，并评估支架的热性能和力学性能的变化。由于添加HA，未观察到降解温度起始点的变化，然而，当复合制剂中HA含量增加时，共聚物的压缩模量和拉伸模量发生了显著变化。发现共聚物力学性能的变化与构建体中HA浓度密切相关。电子显微镜显示有矿物质成核现象，表面形态发生变化，并且使用能量色散X射线分析证实了表面存在钙和磷。为了表征矿物质对成骨前体细胞附着和成熟的影响，将W20-17细胞接种在HA/共聚物复合材料上。我们证明细胞更多地附着在含HA共聚物的表面，并且它们的增殖速率显著提高。因此，这些发现表明HA/PPF-co-PCL复合支架能够诱导成骨前体细胞成熟，并且有潜力用作骨组织工程中的支架。
