Laboratory for Nano-Bio Probes, Quantitative Biology Center, Riken, Suita, 565-0874, Osaka, Japan.
Small. 2015 Mar 25;11(12):1396-401. doi: 10.1002/smll.201402508. Epub 2014 Dec 12.
To detect single molecules within the optical diffraction limit (< ca. 200 nm), a multicolored imaging technique is developed using Halo-ligand conjugated quantum dots (Halo-QDs; <6 nm in diameter). Using three types of Halo-QDs, multicolored single-molecule fluorescence imaging of GPCR proteins in Dictyostelium cells is achieved.
为了在光学衍射极限(< ca. 200nm)内检测单个分子，我们开发了一种使用 Halo 配体偶联量子点(Halo-QDs; <6nm 直径)的多色成像技术。使用三种类型的 Halo-QD，我们实现了在 Dictyostelium 细胞中 GPCR 蛋白的多色单分子荧光成像。
