用于细胞膜上拥挤 GPCR 蛋白的多色单分子成像的紧凑型光环配体偶联量子点。
Compact halo-ligand-conjugated quantum dots for multicolored single-molecule imaging of overcrowding GPCR proteins on cell membranes.
机构信息
Laboratory for Nano-Bio Probes, Quantitative Biology Center, Riken, Suita, 565-0874, Osaka, Japan.
出版信息
Small. 2015 Mar 25;11(12):1396-401. doi: 10.1002/smll.201402508. Epub 2014 Dec 12.
PMID:25504902
Abstract
To detect single molecules within the optical diffraction limit (< ca. 200 nm), a multicolored imaging technique is developed using Halo-ligand conjugated quantum dots (Halo-QDs; <6 nm in diameter). Using three types of Halo-QDs, multicolored single-molecule fluorescence imaging of GPCR proteins in Dictyostelium cells is achieved.
摘要
为了在光学衍射极限(< ca. 200nm)内检测单个分子，我们开发了一种使用 Halo 配体偶联量子点(Halo-QDs; <6nm 直径)的多色成像技术。使用三种类型的 Halo-QD，我们实现了在 Dictyostelium 细胞中 GPCR 蛋白的多色单分子荧光成像。
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