Portetelle D, Limbach K, Burny A, Mammerickx M, Desmettre P, Riviere M, Zavada J, Paoletti E
Faculty of Agronomy, Gembloux, Belgium.
Vaccine. 1991 Mar;9(3):194-200. doi: 10.1016/0264-410x(91)90153-w.
The bovine leukaemia virus (BLV) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into the HA locus of vaccinia virus (Copenhagen strain), downstream of the vaccinia virus early-late promoter, H6, or a triple promoter element consisting of the promoter for the vaccinia virus H6 gene, the promoter for the cowpox virus A-type inclusion (ATI) gene and the promoter for the vaccinia virus HA gene. Inoculation of rabbits or sheep with the recombinant vaccinia virus coding for gp51 and gp30 or an uncleaved env precursor induced neutralizing antibodies to BLV. These antibodies competed with monoclonal antibodies directed against gp51 epitopes F, G, and H previously shown to be of crucial importance for BLV infection. Seven out of eight sheep vaccinated with the vaccinia recombinants resisted a drastic challenge (1.5 x 10(3) sheep infectious doses) with BLV-infected lymphocytes. These results show that vaccination with BLV env vaccinia recombinants protects sheep against infection with extremely high doses of BLV-infected heterologous lymphocytes.
编码细胞外糖蛋白gp51和跨膜糖蛋白gp30的牛白血病病毒（BLV）包膜基因被克隆到痘苗病毒（哥本哈根株）的HA基因座中，位于痘苗病毒早期-晚期启动子H6或由痘苗病毒H6基因启动子、牛痘病毒A型包涵体（ATI）基因启动子和痘苗病毒HA基因启动子组成的三联启动子元件的下游。用编码gp51和gp30的重组痘苗病毒或未切割的env前体接种兔子或绵羊，可诱导产生针对BLV的中和抗体。这些抗体与先前显示对BLV感染至关重要的针对gp51表位F、G和H的单克隆抗体发生竞争。用痘苗重组体接种的八只绵羊中有七只抵抗了用BLV感染的淋巴细胞进行的剧烈攻击（1.5×10³个绵羊感染剂量）。这些结果表明，用BLV env痘苗重组体进行疫苗接种可保护绵羊免受极高剂量的BLV感染的异源淋巴细胞的感染。
