Sack R B, Miller C E
J Bacteriol. 1969 Sep;99(3):688-95. doi: 10.1128/jb.99.3.688-695.1969.
Germ-free mice inoculated with Vibrio cholerae became colonized with vibrios throughout the gastrointestinal tract, but they did not become ill. High vibrio concentrations in the feces were observed throughout the 3 months of observation in spite of the presence of serum antibody. Reciprocal conversions of both Inaba and Ogawa serotypes occurred regularly after inoculation and could be correlated temporally with the appearance of serum-agglutinating antibody. Both of these smooth serotype were later progressively replaced by rough vibrios. Rough to smooth serotype reversions were also found in mice inoculated with a rough vibrio strain. Studies employing immunosuppression and selective vaccination suggested that such serotypic changes were a result of the selective effect of antibody within the intestinal lumen.
接种霍乱弧菌的无菌小鼠在整个胃肠道内都被弧菌定植，但它们并未发病。尽管存在血清抗体，但在整个3个月的观察期内，粪便中都观察到了高浓度的弧菌。接种后，稻叶型和小川型血清型定期发生相互转换，并且在时间上与血清凝集抗体的出现相关。这两种光滑血清型后来逐渐被粗糙型弧菌取代。在接种粗糙型弧菌菌株的小鼠中也发现了粗糙型向光滑型血清型的逆转。采用免疫抑制和选择性疫苗接种的研究表明，这种血清型变化是肠腔内抗体选择性作用的结果。
