Cheng Gong, Hao Si-Jie, Yu Xu, Zheng Si-Yang
Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Lab Chip. 2015 Feb 7;15(3):650-4. doi: 10.1039/c4lc01165a.
A novel microfluidic protein digestion system with a nanostructured and bioactive inner surface was constructed by an easy biomimetic self-assembly strategy for rapid and effective proteolysis in 2 minutes, which is faster than the conventional overnight digestion methods. It is expected that this work would contribute to rapid online digestion in future high-throughput proteomics.
通过一种简便的仿生自组装策略构建了一种具有纳米结构和生物活性内表面的新型微流控蛋白质消化系统，可在2分钟内实现快速有效的蛋白水解，这比传统的过夜消化方法更快。预计这项工作将有助于未来高通量蛋白质组学中的快速在线消化。
