Takahashi M, Fujimoto Y
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Biochem Biophys Res Commun. 1989 Sep 15;163(2):1182-8. doi: 10.1016/0006-291x(89)92345-0.
A Balb/c mouse was immunized with chick synaptic plasma membranes and monoclonal antibodies were produced by fusion of spleen cells with NS-1 mouse myeloma cells. One antibody, MAC-L1, immunoprecipitated more than 90% of the [3H]PN200-110-labeled calcium channels but only 20% of the omega -conotoxin receptor solubilized from the chick brain membranes. Thus possibly, a certain portion of the omega -conotoxin receptor in the chick brain is a dihydropyridine-sensitive calcium channel. By the specific immunoprecipitation of 125I-labeled proteins, two large polypeptides of 193kDa and 130kDa under reducing conditions were identified as the major components of the calcium channel.
用鸡突触质膜免疫一只Balb/c小鼠，通过脾细胞与NS-1小鼠骨髓瘤细胞融合产生单克隆抗体。一种抗体MAC-L1免疫沉淀了超过90%的[3H]PN200-110标记的钙通道，但仅沉淀了从鸡脑膜中溶解的ω-芋螺毒素受体的20%。因此，鸡脑中的ω-芋螺毒素受体的某一部分可能是对二氢吡啶敏感的钙通道。通过对125I标记蛋白的特异性免疫沉淀，在还原条件下鉴定出193kDa和130kDa的两种大的多肽是钙通道的主要成分。
