Saisu H, Ibaraki K, Yamaguchi T, Sekine Y, Abe T
Department of Neurochemistry, Niigata University, Japan.
Biochem Biophys Res Commun. 1991 Nov 27;181(1):59-66. doi: 10.1016/s0006-291x(05)81381-6.
Two monoclonal antibodies raised against brain synaptic membranes immunoprecipitated significant fractions of the brain omega-conotoxin receptor (probable omega-conotoxin-sensitive calcium channels) solubilized with digitonin. These antibodies recognized different proteins of 36 kDa and 28 kDa, respectively. Immunoblot analysis of fractions obtained by sucrose gradient centrifugation suggested that these two proteins were not subunits of the omega-conotoxin receptor but were bound to it. These proteins were found to be conserved at least from an amphibian to mammals, and to be present in the nervous system and adrenal medulla among the tissues examined.
两种针对脑突触膜产生的单克隆抗体免疫沉淀了相当一部分用洋地黄皂苷溶解的脑ω-芋螺毒素受体（可能是对ω-芋螺毒素敏感的钙通道）。这些抗体分别识别36 kDa和28 kDa的不同蛋白质。通过蔗糖梯度离心获得的组分的免疫印迹分析表明，这两种蛋白质不是ω-芋螺毒素受体的亚基，而是与其结合。发现这些蛋白质至少从两栖动物到哺乳动物都保守，并且在所检查的组织中存在于神经系统和肾上腺髓质中。
