Faia-Torres Ana B, Goren Tolga, Ihalainen Teemu O, Guimond-Lischer Stefanie, Charnley Mirren, Rottmar Markus, Maniura-Weber Katharina, Spencer Nicholas D, Reis Rui L, Textor Marcus, Neves Nuno M
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho , 4806-909 Caldas das Taipas, Guimarães, Portugal.
ACS Appl Mater Interfaces. 2015 Feb 4;7(4):2367-75. doi: 10.1021/am506951c. Epub 2015 Jan 23.
The success of synthetic bone implants requires good interface between the material and the host tissue. To study the biological relevance of fibronectin (FN) density on the osteogenic commitment of human bone marrow mesenchymal stem cells (hBM-MSCs), human FN was adsorbed in a linear density gradient on the surface of PCL. The evolution of the osteogenic markers alkaline phosphatase and collagen 1 alpha 1 was monitored by immunohistochemistry, and the cytoskeletal organization and the cell-derived FN were assessed. The functional analysis of the gradient revealed that the lower FN-density elicited stronger osteogenic expression and higher cytoskeleton spreading, hallmarks of the stem cell commitment to the osteoblastic lineage. The identification of the optimal FN density regime for the osteogenic commitment of hBM-MSCs presents a simple and versatile strategy to significantly enhance the surface properties of polycaprolactone as a paradigm for other synthetic polymers intended for bone-related applications.
合成骨植入物的成功需要材料与宿主组织之间良好的界面。为了研究纤连蛋白（FN）密度对人骨髓间充质干细胞（hBM-MSCs）成骨定向的生物学相关性，将人FN以线性密度梯度吸附在聚己内酯（PCL）表面。通过免疫组织化学监测成骨标志物碱性磷酸酶和胶原蛋白1α1的演变，并评估细胞骨架组织和细胞衍生的FN。对梯度的功能分析表明，较低的FN密度引发更强的成骨表达和更高的细胞骨架铺展，这是干细胞向成骨细胞谱系定向的标志。确定hBM-MSCs成骨定向的最佳FN密度范围，为显著增强聚己内酯的表面性能提供了一种简单且通用的策略，可作为其他用于骨相关应用的合成聚合物的范例。
