Sang Hui, Yao Shutong, Zhang Liying, Li Xiuhong, Yang Nana, Zhao Jianxiang, Zhao Li, Si Yanhong, Zhang Ying, Lv Xiaohong, Xue Yazhuo, Qin Shucun
Institute of Atherosclerosis, Key Laboratory of Atherosclerosis in the Universities of Shandong (H.S., S.Y., N.Y., L.Z., Y.S., Y.Z., S.Q.), Taishan Medical University, Taian 271000, China; Institute of Basic Medicine (H.S., S.Y., S.Q.), Taishan Medical University, Taian 271000, China; Institute of Nursing (L.Z., Y.X.), Taishan Medical University, Taian 271016, China; Taian Mental Diseases Hospital (X.L.), Taian 271000, China; Clinical Laboratory (J.Z.), The Affiliated Hospital, Taishan Medical University, Taian 271000, China; The Affiliated Hospital of Chengde Medical University (L.Z.), Chengde Medical University, Chengde, Hebei 067000, China; Department of Endocrinology (X.L.), The Central Hospital of Taian, Taian 271000, China.
J Clin Endocrinol Metab. 2015 Mar;100(3):870-9. doi: 10.1210/jc.2014-2979. Epub 2014 Dec 16.
Metabolic syndrome (MetS) is a constellation of cardiovascular risk factors, including central obesity, dysglycemia, hypertension, and dyslipidemia. The anti-inflammatory properties of high density lipoprotein (HDL) can be compromised in MetS. Exercise is recognized as an important factor in the prevention and treatment of MetS.
This study was designed to investigate whether walk/run training without any specific diet could enhance anti-inflammation capacity of HDL from MetS patients.
This was a case control study.
The study was conducted in a Zhoudian community, Taian.
Thirty nine patients with MetS were recruited and divided into a control group (n = 12) remaining in an untrained state and exercise group (n = 27) performing a 10-week walk/run training program.
The anti-inflammation capacities of HDL3 (HDL subfractions) from MetS patients with or without exercise were investigated by co-incubating with TNF- α-injured endothelial cells in vitro.
The training did not influence serum lipoprotein level in MetS patients and cholesterol efflux capacity of circulating HDL. However, walk/run training increased paraoxonase-1 (PON1) activity and decreased the levels of malondialdehyde in either serum or isolated HDL from MetS patients prominently. More importantly, HDL3 isolated from MetS patients with 10 weeks training protected endothelial cells against tumor necrosis factor-a (TNF-a) -induced injury, decreased monocyte chemotactic protein-1 levels in media and vascular cell adhesion molecule-1 expression markedly. Furthermore, HDL3 isolated from MetS patients with walk/run training inhibited the TNF-á-induced monocyte adhesion to endothelial cells and obviously increased nitric oxide production by activating endothelial nitric oxide synthase.
Walk/run training leads to a significant improvement in HDL anti-inflammation capacity in subjects with MetS without restricted diet, the mechanism underlying which at least partially is due to increased PON1 activity in HDL, NO production, and eNOS expression in endothelial cells.
代谢综合征（MetS）是一组心血管危险因素，包括中心性肥胖、血糖异常、高血压和血脂异常。高密度脂蛋白（HDL）的抗炎特性在代谢综合征中可能会受到损害。运动被认为是预防和治疗代谢综合征的一个重要因素。
本研究旨在调查在没有任何特定饮食的情况下，步行/跑步训练是否能增强代谢综合征患者HDL的抗炎能力。
这是一项病例对照研究。
该研究在泰安的周店社区进行。
招募了39例代谢综合征患者，分为对照组（n = 12），保持未训练状态；运动组（n = 27），进行为期10周的步行/跑步训练计划。
通过在体外与肿瘤坏死因子-α（TNF-α）损伤的内皮细胞共同孵育，研究有或没有运动的代谢综合征患者HDL3（HDL亚组分）的抗炎能力。
训练并未影响代谢综合征患者的血清脂蛋白水平和循环HDL的胆固醇流出能力。然而，步行/跑步训练显著提高了代谢综合征患者血清或分离的HDL中对氧磷酶-1（PON1）的活性，并降低了丙二醛水平。更重要的是，从经过10周训练的代谢综合征患者中分离出的HDL3可保护内皮细胞免受肿瘤坏死因子-α（TNF-α）诱导的损伤，显著降低培养基中单核细胞趋化蛋白-1水平和血管细胞黏附分子-1表达。此外，从进行步行/跑步训练的代谢综合征患者中分离出的HDL3抑制了TNF-α诱导的单核细胞与内皮细胞的黏附，并通过激活内皮型一氧化氮合酶明显增加了一氧化氮的产生。
在没有严格饮食限制的情况下，步行/跑步训练可使代谢综合征患者的HDL抗炎能力得到显著改善，其潜在机制至少部分归因于HDL中PON1活性增加、一氧化氮产生以及内皮细胞中eNOS表达增加。
