Hucklebridge F H, Hudspith B N, Muhamed J, Lydyard P M, Brostoff J
Polytechnic of Central London, School of Paramedical Studies, England.
Brain Behav Immun. 1989 Jun;3(2):183-9. doi: 10.1016/0889-1591(89)90019-6.
The influence of methionine-enkephalin (Met-Enk) on in vitro proliferation of human peripheral lymphocytes was investigated. Met-Enk, in the concentration range 10(-12) to 10(-4) M enhanced the proliferative response to suboptimal concentrations of concanavalin A of human peripheral lymphocytes and to cells in the absence of mitogen. The response to Met-Enk in the absence of mitogen was not influenced by the presence of fetal calf serum: similar levels of enhancement were seen in cultures supplemented with 10% autologous serum. Enhancement of proliferation was blocked in a concentration-dependent manner by both naloxone and the delta specific antagonist ICI-174864. The sensitivity to the antagonistic influence of ICI-174864 suggests strongly that the stimulatory influence of Met-Enk on human lymphocyte proliferation in the absence of mitogen is mediated via the delta-opioid receptor.
研究了甲硫氨酸脑啡肽（Met-Enk）对人外周血淋巴细胞体外增殖的影响。浓度范围为10^(-12)至10^(-4) M的Met-Enk增强了人外周血淋巴细胞对次优浓度伴刀豆球蛋白A以及无丝裂原情况下细胞的增殖反应。在无丝裂原时对Met-Enk的反应不受胎牛血清存在的影响：在补充10%自体血清的培养物中观察到类似程度的增强。纳洛酮和δ特异性拮抗剂ICI-174864均以浓度依赖性方式阻断增殖增强。对ICI-174864拮抗作用的敏感性强烈表明，在无丝裂原时Met-Enk对人淋巴细胞增殖的刺激作用是通过δ阿片受体介导的。
