Rehbein Peter, Schwalbe Harald
Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main, Germany.
Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main, Germany.
Protein Expr Purif. 2015 Jun;110:1-6. doi: 10.1016/j.pep.2014.12.006. Epub 2014 Dec 13.
Quantitative analysis of electrophoresis gels is an important part in molecular cloning, as well as in protein expression and purification. Parallel quantifications in yield and purity can be most conveniently obtained from densitometric analysis. This communication reports a comprehensive, reliable and simple protocol for gel quantification and documentation, applicable for single samples and with special features for protein expression screens. As major component of the protocol, the fully annotated code of a proprietary open source computer program for semi-automatic densitometric quantification of digitized electrophoresis gels is disclosed. The program ("GelQuant") is implemented for the C-based macro-language of the widespread integrated development environment of IGOR Pro.
电泳凝胶的定量分析是分子克隆以及蛋白质表达与纯化中的重要环节。通过光密度分析能够最便捷地同时获得产量和纯度的平行定量结果。本文介绍了一种全面、可靠且简单的凝胶定量与记录方案，适用于单个样品，尤其适用于蛋白质表达筛选。作为该方案的主要组成部分，公开了一个专有的开源计算机程序的完整注释代码，用于对数字化电泳凝胶进行半自动光密度定量分析。该程序（“GelQuant”）是为广泛使用的IGOR Pro集成开发环境基于C语言的宏语言而实现的。
