超氧阴离子增强血小板黏附和聚集。
Superoxide anions enhance platelet adhesion and aggregation.
作者信息
Salvemini D, de Nucci G, Sneddon J M, Vane J R
机构信息
William Harvey Research Institute, St Bartholomew's Hospital Medical College, London.
出版信息
Br J Pharmacol. 1989 Aug;97(4):1145-50. doi: 10.1111/j.1476-5381.1989.tb12572.x.
Abstract
- Superoxide dismutase (SOD, 60 u ml-1) or ferricytochrome c (70 microM) significantly inhibited thrombin-stimulated platelet adhesion to gelatin-coated plastic, whereas catalase (1000 u ml-1) or mannitol (1 mM) had no effect. 2. The platelet aggregation induced by low concentrations of thrombin (causing less than 45% maximal change in light transmission) was inhibited by SOD. Catalase or mannitol had no effect on platelet aggregation. 3. Pyrogallol (an O2- generator) enhanced both platelet adhesion to gelatin-coated plastic and platelet aggregation induced by thrombin; this enhancement was neutralized by SOD. 4. These results indicate that O2- increase both platelet adhesion and aggregation, whereas other free radicals such as hydrogen peroxide or hydroxyl radicals are not involved.
摘要
- 超氧化物歧化酶（SOD，60单位/毫升）或铁细胞色素c（70微摩尔）可显著抑制凝血酶刺激的血小板与明胶包被塑料的黏附，而过氧化氢酶（1000单位/毫升）或甘露醇（1毫摩尔）则无此作用。2. 低浓度凝血酶诱导的血小板聚集（导致光透射率最大变化小于45%）可被SOD抑制。过氧化氢酶或甘露醇对血小板聚集无影响。3. 邻苯三酚（一种超氧阴离子生成剂）可增强血小板与明胶包被塑料的黏附以及凝血酶诱导的血小板聚集；这种增强作用可被SOD中和。4. 这些结果表明，超氧阴离子可增加血小板的黏附和聚集，而其他自由基如过氧化氢或羟自由基则未参与其中。
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