Kovalenko Elena I, Boyko Anna A, Semenkov Victor F, Lutsenko Gennady V, Grechikhina Maria V, Kanevskiy Leonid M, Azhikina Tatyana L, Telford William G, Sapozhnikov Alexander M
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.
Centre of Gerontology of Russian State Medical University, Moscow, Russia.
Oncotarget. 2014 Dec 15;5(23):11800-12. doi: 10.18632/oncotarget.2856.
ROS production and intracellular HSP70 levels were measured in human neutrophils for three age groups: young (20-59 years), elders (60-89 years) and nonagenarians (90 years and older). Elders showed higher levels of spontaneous intracellular ROS content compared with young and nonagenarian groups, which had similar intracellular ROS levels. Zymosan-induced (non-spontaneous) extracellular ROS levels were also similar for young and nonagenarians but were lower in elders. However, spontaneous extracellular ROS production increased continuously with age. Correlation analysis revealed positive relationships between HSP70 levels and zymosan-stimulated ROS production in the elder group. This was consistent with a promoting role for HSP70 in ROS-associated neutrophils response to pathogens. No positive correlation between ROS production and intracellular HSP70 levels was found for groups of young people and nonagenarians. In contrast, significant negative correlations of some ROS and HSP70 characteriscics were found for neutrophils from young people and nonagenarians. The observed difference in ROS and HSP70 correlations in elders and nonagenarians might be associated with an increased risk of mortality in older individuals less than 90 years old.
在三个年龄组的人类中性粒细胞中测量了活性氧（ROS）的产生和细胞内热休克蛋白70（HSP70）水平：年轻人（20 - 59岁）、老年人（60 - 89岁）和九旬老人（90岁及以上）。与细胞内ROS水平相似的年轻人和九旬老人组相比，老年人的自发细胞内ROS含量更高。酵母聚糖诱导的（非自发的）细胞外ROS水平在年轻人和九旬老人中也相似，但在老年人中较低。然而，自发的细胞外ROS产生随年龄持续增加。相关性分析显示，老年组中HSP70水平与酵母聚糖刺激的ROS产生之间存在正相关关系。这与HSP70在ROS相关的中性粒细胞对病原体的反应中起促进作用一致。在年轻人和九旬老人组中，未发现ROS产生与细胞内HSP70水平之间存在正相关。相反，在年轻人和九旬老人的中性粒细胞中发现了一些ROS和HSP70特征之间的显著负相关。在老年人和九旬老人中观察到的ROS和HSP70相关性差异可能与90岁以下老年人死亡风险增加有关。
