Tang Jun, Xiao Ping, Luo Xi, Bai Jing, Xia Wentong, Chen Weixiong, Li Jian, Yu Qingqing, Shi Sisi, Xu Yuxia, Mou Zhonglin, Wang Yuejian, Li Huabin
Department of Otolaryngology, The First People's Hospital of Foshan City, Foshan, China.
Am J Rhinol Allergy. 2014 Nov-Dec;28(6):197-201. doi: 10.2500/ajra.2014.28.4088.
IL-22 regulates various processes and has been linked to diverse effects. However, the importance of IL-22 in the pathogenesis of allergic rhinitis (AR) remains poorly understood. This study sought to evaluate the levels of IL-22 and IL-17A in AR patients and their association with clinical severity ofN AR.
Thirty-six AR patients and 22 normal controls were enrolled in this study. The frequencies of IL-22(+), IL-17A(+), and IL-9(+) T helper (Th) cells in peripheral blood of AR patients and normal controls were examined by flow cytometry. Serum levels of IL-22 and IL-17A in AR patients and normal controls were determined by ELISA. The clinical relevance of the percentages of IL-22(+) and IL-17A(+) Th cells as well as serum IL-22 and IL-17A levels were evaluated.
The frequencies of IL-22(+) and IL-17A(+) Th cells, but not IL-9(+) Th cells, were significantly increased compared with those in normal controls (p < 0.05). Frequencies of IL-22(+) and IL-17A(+) Th cells in peripheral blood of AR patients significantly correlated with visual analog scale scores of nasal symptoms (nasal congestion and rhinorrhea; p < 0.05). Moreover, the serum levels of IL-22 and IL-17A were significantly increased compared with those in normal controls (p < 0.05) and significantly correlated with the levels of Dermatophagoides pteronyssinus and Dermatophagoides farinae specific IgE in AR patients.
Our findings suggested that IL-22 as well as IL-17A may play an important role in the regulation of Th2-skewed inflammation in AR patients.
白细胞介素-22（IL-22）调节多种生理过程，并与多种效应相关。然而，IL-22在变应性鼻炎（AR）发病机制中的重要性仍知之甚少。本研究旨在评估AR患者中IL-22和IL-17A的水平及其与AR临床严重程度的关系。
本研究纳入了36例AR患者和22例正常对照。采用流式细胞术检测AR患者和正常对照外周血中IL-22(+)、IL-17A(+)和IL-9(+)辅助性T（Th）细胞的频率。采用酶联免疫吸附测定法（ELISA）测定AR患者和正常对照血清中IL-22和IL-17A的水平。评估IL-22(+)和IL-17A(+) Th细胞百分比以及血清IL-22和IL-17A水平的临床相关性。
与正常对照相比，IL-22(+)和IL-17A(+) Th细胞的频率显著增加，但IL-9(+) Th细胞的频率无显著变化（p < 0.05）。AR患者外周血中IL-22(+)和IL-17A(+) Th细胞的频率与鼻症状（鼻塞和流涕）的视觉模拟量表评分显著相关（p < 0.05）。此外，与正常对照相比，AR患者血清中IL-22和IL-17A的水平显著升高（p < 0.05），且与AR患者中屋尘螨和粉尘螨特异性IgE的水平显著相关。
我们的研究结果表明，IL-22以及IL-17A可能在AR患者Th2偏向性炎症的调节中起重要作用。
