Allergy Immunology Department, Tepecik Training and Research Hospital, Health Sciences University, Izmir, Turkey.
Department of Biochemistry, Katip Celebi University, Izmir, Turkey.
Dis Markers. 2018 Mar 6;2018:9131432. doi: 10.1155/2018/9131432. eCollection 2018.
We aimed in our study to research the role of new cytokines such as IL-35, IL-22, and IL-17 that may form a target for novel treatment approaches.
IL-10, IL-17, TGF-, IFN-, IL-22, and IL-35 serum levels of allergic rhinitis (AR) patients were measured using ELISA method. Allergic sensitization was demonstrated by the skin prick test. Patients only with olive tree sensitivity were evaluated for seasonal AR (SAR). Patients only with mite sensitivity were included in the study for perennial AR (PAR). AR clinic severity was demonstrated by the nasal symptom scores (NSS).
In total, 65 AR patients (patient group), having 31 PAR and 34 SAR patients, and 31 healthy individuals (control group) participated in the study. Cytokine levels between the patient group and the control group were compared; IL-17 ( = 0.038), IL-22 ( = 0.001), and TGF- ( = 0.031) were detected as high in the patient group, and IFN- ( < 0.001) was detected as low in the patient group. When correlation analysis was made between age, gender, prick test result, NSS, AR duration, and cytokine levels in the patient group, a negative correlation was detected only between IFN- ( = 0.032/ = -0.266) level and NSS.
Accompanied by the literature information, these results made us think that T cell subgroups and cytokines have an important role in AR immunopathogenesis. It is thought that future studies to be conducted relating to this subject will form new targets in treatment.
我们旨在本研究中研究新的细胞因子（如 IL-35、IL-22 和 IL-17）的作用，这些细胞因子可能成为新的治疗方法的靶点。
采用 ELISA 法测定过敏性鼻炎（AR）患者血清中 IL-10、IL-17、TGF-、IFN-、IL-22 和 IL-35 水平。皮肤点刺试验显示过敏敏化。仅对橄榄树敏感的患者进行季节性 AR（SAR）评估。仅对螨敏感的患者纳入研究进行常年性 AR（PAR）。鼻症状评分（NSS）显示 AR 临床严重程度。
共有 65 名 AR 患者（患者组），其中 31 名 PAR 患者和 34 名 SAR 患者，31 名健康个体（对照组）参加了研究。比较患者组和对照组的细胞因子水平；IL-17（=0.038）、IL-22（=0.001）和 TGF-（=0.031）在患者组中较高，IFN-（<0.001）在患者组中较低。在患者组中，当年龄、性别、点刺试验结果、NSS、AR 持续时间和细胞因子水平之间进行相关分析时，仅 IFN-（=0.032/=-0.266）水平与 NSS 呈负相关。
结合文献信息，这些结果使我们认为 T 细胞亚群和细胞因子在 AR 免疫发病机制中起重要作用。据认为，未来将开展的相关研究将为治疗形成新的靶点。
