Shahsavan Shaghayegh, Pirayesh Ashkan, Samani Omid Zargari, Shirzad Hedayatollah, Zamani Mohamad Ali, Amani Soroush, Kazemi Seyyedeh Maryam, Moghni Mandana, Deris Fatemeh, Bageri Nader, Salimzadeh Loghman, Tavakoli Ghadir, Arjenaki Mostafa Gholami
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Am J Otolaryngol. 2019 Mar-Apr;40(2):173-178. doi: 10.1016/j.amjoto.2018.12.009. Epub 2018 Dec 18.
Several reactions leading to numerous effects are regulated by IL-22. However, the relationship between IL-22 and immunopathogensis of allergic rhinitis (AR) has been rarely investigated. The aim of the present study was to investigate the levels of IL-22 and IL-17A in AR patients and their association with clinical severity of persistent allergic rhinitis (PAR).
Thirty mild persistent allergic rhinitis (M PAR) patients, thirty moderate/severe persistent allergic rhinitis (M/S PAR) patients, and thirty healthy controls were enrolled in this study. Local production of IL-22 and IL-17A in PAR patients and healthy controls' nasal mucosa was examined by immunohistochemistry (IHC) and real-time polymerase chain reaction (RT-PCR) techniques. Serum levels of IL-22, IL-17A, specific immunoglobulin E (sIgE), and total IgE (tIgE) in PAR patients and healthy controls were determined by ELISA. In addition, blood eosinophil, nasal eosinophils per field, and total nasal syndrome score (TNSS) were also assessed.
In comparison with healthy controls, production of IL-22 and IL-17A in M/S PAR patients increased significantly. Furthermore, serum levels as well as the mean number of IL-22 and IL-17A cells in nasal mucosa correlated with sIgE, nasal eosinophil count, and TNSS.
The results of the present study provide the first evidence that local production of IL-22 might be expressed in PAR patients. The expression of IL-22 and IL-17A, and their correlations with clinical parameters in PAR patients suggest the role of these cytokines in the events involved in the development of PAR.
白细胞介素-22（IL-22）可调节多种反应并产生众多效应。然而，IL-22与变应性鼻炎（AR）免疫发病机制之间的关系鲜有研究。本研究旨在探讨AR患者中IL-22和IL-17A的水平及其与持续性变应性鼻炎（PAR）临床严重程度的关联。
本研究纳入30例轻度持续性变应性鼻炎（M PAR）患者、30例中重度持续性变应性鼻炎（M/S PAR）患者和30例健康对照。采用免疫组织化学（IHC）和实时聚合酶链反应（RT-PCR）技术检测PAR患者和健康对照鼻黏膜中IL-22和IL-17A的局部产生情况。采用酶联免疫吸附测定（ELISA）法测定PAR患者和健康对照血清中IL-22、IL-17A、特异性免疫球蛋白E（sIgE）和总免疫球蛋白E（tIgE）的水平。此外，还评估了血液嗜酸性粒细胞、每视野鼻嗜酸性粒细胞以及总鼻症状评分（TNSS）。
与健康对照相比，M/S PAR患者中IL-22和IL-17A的产生显著增加。此外，血清水平以及鼻黏膜中IL-22和IL-17A细胞的平均数与sIgE、鼻嗜酸性粒细胞计数和TNSS相关。
本研究结果首次证明PAR患者鼻黏膜中可能存在IL-22的局部产生。PAR患者中IL-22和IL-17A的表达及其与临床参数的相关性提示这些细胞因子在PAR发生发展过程中的作用。
