Liu Ying, Li Naijing, Zhou Ling, Li Qing, Li Wei
College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110004, Liaoning, China.
Cent Nerv Syst Agents Med Chem. 2014;14(2):113-20. doi: 10.2174/1871524915666141216161246.
Alzheimer's disease (AD) is the main cause of gradual cognitive impairment in elderly individuals. This highlights the need of obtaining biomarkers to identify features that are different among mild cognitive impairment (MCI), AD and cognitively normal (CN) individuals.
Ultra-performance liquid chromatography (UPLC)/mass spectrometry (MS) was employed to find the metabolic changes in plasma samples obtained from AD, MCI and CN individuals. Based on principal component analysis (PCA), the metabolic differences among AD, MCI and CN subjects were identified.
The PCA of UPLC/MS spectra indicated metabolic differences among AD, MCI and CN subjects. The peak intensities of progesterone, lysophos- phatidylcholines (LPCs), tryptophan, L-phenylalanine, dihydrosphingosine and phytosphingosine in the plasma of the MCI and AD subjects were significantly different from the CN subjects. Furthermore, the peak intensities of tryptophan, LPCs, dihydrosphingosine in the plasma of the AD subjects were significantly lower compared to the MCI subjects.
Our data provide a link between metabolite imbalance and AD, and suggest that metabolomics can be used to reveal the early disease mechanisms occurred in the progression from CN to MCI and AD.
阿尔茨海默病（AD）是老年人渐进性认知障碍的主要原因。这凸显了获取生物标志物以识别轻度认知障碍（MCI）、AD和认知正常（CN）个体之间不同特征的必要性。
采用超高效液相色谱（UPLC）/质谱（MS）技术，寻找从AD、MCI和CN个体获取的血浆样本中的代谢变化。基于主成分分析（PCA），确定AD、MCI和CN受试者之间的代谢差异。
UPLC/MS光谱的PCA表明AD、MCI和CN受试者之间存在代谢差异。MCI和AD受试者血浆中孕酮、溶血磷脂酰胆碱（LPC）、色氨酸、L-苯丙氨酸、二氢鞘氨醇和植物鞘氨醇的峰强度与CN受试者显著不同。此外，AD受试者血浆中色氨酸、LPC、二氢鞘氨醇的峰强度与MCI受试者相比显著降低。
我们的数据提供了代谢物失衡与AD之间的联系，并表明代谢组学可用于揭示从CN进展到MCI和AD过程中发生的早期疾病机制。
